[Physiofaculty] Special Equipment: Protein Printing and Quantitative Phase

[Brzostowski, Joseph (NIH/NIAID) [E]]

Dear Faculty and TAs.

I am writing to remind you that we have two really interesting peripherals that will be installed on widefield systems for the first session and will be with us for the full course.

On the Nikon widefield we will have the PRIMO from Alveolab.

Primo is an innovative solution for printing proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be printed is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.

Please visit:
http://www.alveolelab.com


One of the Zeiss widefield scopes will be set up for quantitative phase imaging from Phi Optics.
Phi Optics patented technology – Spatial Light Interference Microscopy (SLIM) – employs optical interferometry for extreme sensitivity to structure and dynamics. Phi Optics implements SLIM as an add-on to all major brand optical microscopes (10X to 100X magnifications) and overlays with fluorescence. It connects via C-mount and uses the white-light illumination source of the microscope.

Please visit:
http://phioptics.com/technology/overview/

Looking forward to seeing old friends and meeting new ones in the weeks to come !

Joe

--
Joseph Brzostowski, Ph.D.
LIG Imaging Facility, Chief
National Institutes of Health/NIAID
12441 Parklawn Dr. Twin II/Rm. 200 E
Rockville, MD 20852

Mobile: 240-599-6364
Cu Wire: 301-761-5024
Fax: 301-402-0259

jb363a at nih.gov
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