<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div apple-content-edited="true">Hello Physiology!
</div><div apple-content-edited="true"><br></div><div apple-content-edited="true">In the message Yesterday I mentioned that we can purchase anything from Promega for FREE. </div><div apple-content-edited="true"><br></div><div apple-content-edited="true">A couple of weeks ago I sent out a message to the Faculty suggesting they consider using Promega <a href="http://www.promega.com/products/pm/halotag-technology/halotag-technology/">Halo tag</a> constructs. You can order <a href="http://www.promega.com/FindMyGene/search.aspx">any human or mouse protein with a halo tag</a> You can then use any of their cell permeable or impermeable ligands to visualize or purify your protein. But you need to order them soon because there is a lag time for these constructs to be made. (Free). A couple people from the JLS group ordered these last year, but I think other groups might have interest as well.</div><div apple-content-edited="true"><br></div><div apple-content-edited="true">Promega offers several other products that might be of interest. They sent the following information for people interested in cell health assays: </div>
<div><br><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple" style="word-wrap: break-word;-webkit-nbsp-mode: space;-webkit-line-break: after-white-space"><div class="WordSection1"><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> Attached is a PDF Cell Health Assay guide, which includes the majority of our cell
health and metabolism assays. Many of these can be multiplexed. We’d be happy to discuss with anyone who is interested.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> </span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">The main assay not included is our new
<a href="http://www.promega.com/products/cell-health-and-metabolism/cell-viability-assays/realtime-glo-monitor-cell-viability-in-real-time/">
RealTime-Glo™ MT Cell Viability Assay</a>. It is a counterpart to our CellTox™ Green Cytotoxicity Assay. Both allow for monitoring cell health in real time.
<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> </span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Other assays are:<o:p></o:p></span></p><p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span style="font-size:11.0pt;font-family:Symbol;color:#1F497D"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><a href="http://www.promega.com/products/cell-health-and-metabolism/cell-viability-assays/celltiter-glo-3d-microtissue-cultures/">CellTiter-Glo® 3D</a>
for measuring cell viability in 3D microtissues<o:p></o:p></span></p><p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span style="font-size:11.0pt;font-family:Symbol;color:#1F497D"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><a href="http://www.promega.com/products/pm/viral-toxglo/">Viral Tox-Glo™ Assay</a> for determining viral-induced cytopathic effects (CPE) in host
cells caused by lytic virions<o:p></o:p></span></p><p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span style="font-size:11.0pt;font-family:Symbol;color:#1F497D"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><a href="http://www.promega.com/products/cell-health-and-metabolism/mitochondrial-function-assays/">Mitochondrial Tox-Glo™ Assay</a> for predicting
potential mitochondrial dysfunction as a result of xenobiotic exposure<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> </span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> If you have technical questions, please feel free to reach out to:</span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> </span></p><p class="MsoNormal" style="background:white"><b><span style="font-size: 9pt; font-family: Arial, sans-serif;">Dee Czarniecki<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-bottom:1.0pt;background:white"><span style="font-size: 9pt; font-family: Arial, sans-serif;">Scientific Instructional Designer<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom:1.0pt;background:white"><span style="font-size: 9pt; font-family: Arial, sans-serif;">Promega Corporation<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom:1.0pt;background:white"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:#7F7F7F">tel</span><span style="font-size: 8pt; font-family: Arial, sans-serif;"> 608 274 4330
</span><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:#7F7F7F">fax</span><span style="font-size: 8pt; font-family: Arial, sans-serif;"> 608 277 2677
<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom:12.0pt;background:white"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:#226A85"><a href="mailto:dee.czarniecki@promega.com"><span style="color:blue">dee.czarniecki@promega.com</span></a></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#226A85"><o:p></o:p></span></p><p class="MsoNormal"><br></p><div><div><div><div><div><div><div>
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