<div dir="ltr">Dear phyio faculty.<div><br></div><div>I will be bringing a few extra pieces of equipment into the course, and figured a few of you (Nicole, Allison, Jennifer, and Wallace especially) might be interested.</div><div>(so, let me know if these are of use, and how we could synergize / prepare).</div><div><br></div><div><br></div><div>1. I will be bringing the updated version of the electroporator from Bulldog bio. This machine apparently can get DNA into many creatures, including Diatoms, many bacteria etc.</div><div>So, any of you into exotic bacteria /protists, euks, if you have constructs to test transfection, please bring them along. I will have broad range promoters for the prokaryotic kingdom.</div><div><br></div><div>2. I also will be bringing a phase holographic imaging scope, this gets Z information from the difference in the index of refraction of cells vs media. This gives volume, density. Mazing for cell tracking of motile cells, and also and large surface imaging of communities. (see the attached image of a growing bacterial colony front).</div><div><br></div><div><img src="cid:ii_14cdc3e680a44244" alt="Inline image 1" width="562" height="380"><br></div><div><br></div><div><br></div><div>3. Matt Paszek will be coming up to build a Scanning angle Microscope,which gives ~5nm resolution in Z (for fluorescence) with fast time acquisition.</div><div>(his papers attached, if you don't know the technique)</div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br clear="all"><div><div class="gmail_signature"><div dir="ltr"><div><div style="font-family:arial;font-size:small"><span style="font-family:arial,helvetica,sans-serif">Ethan Garner - </span><a href="mailto:egarner@g.harvard.edu" style="font-family:arial,helvetica,sans-serif;color:rgb(17,85,204)" target="_blank">egarner@g.harvard.edu</a></div><div style="font-family:arial;font-size:small"><div><div></div></div><div></div></div><div style="font-family:arial;font-size:small"><font face="arial, helvetica, sans-serif" size="2">Assistant Professor @ </font><span style="font-family:arial,helvetica,sans-serif">Harvard </span></div><div style="font-family:arial;font-size:small"><span style="font-family:arial,helvetica,sans-serif">Molecular and Cellular Biology / Center for Systems Biology</span></div> <div style="font-family:arial;font-size:small"><i><font face="arial, helvetica, sans-serif" size="2">Please contact me via digital means rather than analog.</font></i></div><div style="font-family:arial;font-size:small"><div><span style="font-family:arial,helvetica,sans-serif"><b>To schedule a meeting: </b> </span><a href="http://meetme.so/EthanGarner" style="color:rgb(17,85,204);font-family:arial,helvetica,sans-serif" target="_blank">http://meetme.so/EthanGarner</a></div></div><div style="font-family:arial;font-size:small"><span style="font-family:arial,helvetica,sans-serif"><b>Website</b> </span><a href="http://garnerlab.fas.harvard.edu/" style="color:rgb(17,85,204);font-family:arial,helvetica,sans-serif" target="_blank">http://garnerlab.fas.harvard.edu</a></div></div><div><div><div><font face="arial, helvetica, sans-serif" size="2"><br></font></div></div><div><div><br></div></div></div></div></div></div>
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