<div dir="ltr"><div>Hello Session 3 faculty! </div><div><br></div><div>Just wanted to send out a reminder email with the available microscope systems.</div><div><br></div><div>If you plan to use a system heavily for your rotation and would need training please set up a time with me and I will coordinate it. </div><div><br></div><div>All the systems on our floor are paper signups on the door of the system and sign up time is 10pm for the next day. </div><div><br></div><div>Shared systems in the basement and Lillie, you will need to sign up with PPMS. <b>You can sign up now for next week and I would suggest doing so as these are shared and heavily used by other courses. </b></div><div><br></div><div>PPMS Login:</div><div>go to: <a href="https://ppms.us/mbl/login/?pf=2">https://ppms.us/mbl/login/?pf=2</a></div><div>User: Physiology Student</div><div>Pass: mblpass</div><div><br></div><div><font size="4"><b>Systems Available:</b></font></div><div><br></div><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Widefield<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 1 (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 2 (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 3 (full course) – fully automated with incubation, and<b>PhiOptics </b><b style="font-size:12pt">Quantitative Phase</b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon (full course) – fully automated with incubation, autofocus and <b>PRIMO for protein printing<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE DeltaVision (Sessions 1 – 3) – fully automated with incubation (no CO2)<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) – fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Spinning Disk Confocal<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon/Yokogawa CSUX1 (full course) – fully automated with incubation. With 405 FRAP<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">CREST (Sessions 1 – 3) – partially automated with incubation spinning disk system from BioVision/89 North on an Olympus base run with MetaMorph software. Note, this system only has a very nice 60x water lens. Please see me or Steven for training.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> </p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><span style="font-size:12pt"> </span></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>TIRF<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE DeltaVision (Sessions 1 – 3) – fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) – fully automated with incubation, ring-TIRF version</p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><br></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Super-resolution<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) Structured Illumination (SIM)– fully automated with incubation<b><u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u><br><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Multi-well plate imaging<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Incucyte (full course) – phase, green and red fluorescence. System is housed in a standard 37°C incubator with CO2.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><br></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Microscope systems in basement of Loeb open for the MBL community. All are available for the full course.<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><u></u> <u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Zeiss<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Spinning disk confocal (Yokogawa CSUX1) – fully automated with incubation with FRAP and Photoablation from Rapp Opoto.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><u></u> <u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">880 with Airyscan and FAST – laser scanning confocal with super-res and fast scanning for live cell imaging. Fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">800 – laser scanning confocal fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Widefield system with and injection apparatus.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Nikon<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">STORM 4 – super-resolution TIRF system. Fully automated and will have incubation for standard TIRF experiments. For Session 2 the system will be equipped with the CU 3D to improve 3D STORM acquisitions.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">A1R+ - resonance scanning confocal system. Fully automated with incubation and autofocus.</p><div><br></div>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><table cellpadding="0" cellspacing="0">
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<td rowspan="3"><b>Steven Wilbert</b><br>Research Assistant<br>Microbiology<br>Forsyth Institute <br>585-802-4733 <br></td>
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