<div dir="ltr"><span id="docs-internal-guid-9864a71e-ef21-4a85-8e7d-ca50f93da280"><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Based on responses so far, I have coordinated the following trainings for this Saturday (tomorrow). </span></p><br><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Please let me know which you and your people are interested in attending so that I can convey to the trainee and make sure there are participants. </span></p><br><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">CREST Spinning Disk (dual camera system (green/red), runs metamorph) Training - Steven</span></p><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Sat 1-2pm</span></p><br><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Nikon Spinning Disk Training/Elements Software Intro - John from Nikon</span></p><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Sat 2-3pm</span></p><br><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Zeiss Widefield Training/Zen Software Intro - Jason/Saige from Zeiss</span></p><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Sat 3-4pm</span></p><br><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Zeiss Spinning Disk - Steven</span></p><p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Sat 5-6pm </span></p><br><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">As for the OMX and AiryScan, trainings from the manufacturers will need to be early next week, still coordinating with them. But in the meantime, if you plan to use these right away next week, I can help in getting started. </span></span><br><div><span><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent"><br></span></span></div><div><span><span style="font-size:14.6667px;font-family:Arial;color:rgb(0,0,0);vertical-align:baseline;white-space:pre-wrap;background-color:transparent">Thanks!</span></span></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Fri, Jul 15, 2016 at 9:04 AM, Steven Wilbert <span dir="ltr"><<a href="mailto:swilbert88@gmail.com" target="_blank">swilbert88@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr"><div>Hello Session 3 faculty! </div><div><br></div><div>Just wanted to send out a reminder email with the available microscope systems.</div><div><br></div><div>If you plan to use a system heavily for your rotation and would need training please set up a time with me and I will coordinate it. </div><div><br></div><div>All the systems on our floor are paper signups on the door of the system and sign up time is 10pm for the next day. </div><div><br></div><div>Shared systems in the basement and Lillie, you will need to sign up with PPMS. <b>You can sign up now for next week and I would suggest doing so as these are shared and heavily used by other courses. </b></div><div><br></div><div>PPMS Login:</div><div>go to: <a href="https://ppms.us/mbl/login/?pf=2" target="_blank">https://ppms.us/mbl/login/?pf=2</a></div><div>User: Physiology Student</div><div>Pass: mblpass</div><div><br></div><div><font size="4"><b>Systems Available:</b></font></div><div><br></div><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Widefield<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 1 (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 2 (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Zeiss 3 (full course) – fully automated with incubation, and<b>PhiOptics </b><b style="font-size:12pt">Quantitative Phase</b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon (full course) – fully automated with incubation, autofocus and <b>PRIMO for protein printing<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE DeltaVision (Sessions 1 – 3) – fully automated with incubation (no CO2)<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) – fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Spinning Disk Confocal<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon/Yokogawa CSUX1 (full course) – fully automated with incubation. With 405 FRAP<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">CREST (Sessions 1 – 3) – partially automated with incubation spinning disk system from BioVision/89 North on an Olympus base run with MetaMorph software. Note, this system only has a very nice 60x water lens. Please see me or Steven for training.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> </p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><span style="font-size:12pt"> </span></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>TIRF<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Nikon (full course) – fully automated with incubation and autofocus<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE DeltaVision (Sessions 1 – 3) – fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) – fully automated with incubation, ring-TIRF version</p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><br></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Super-resolution<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">GE OMX (Sessions 1 – 3) Structured Illumination (SIM)– fully automated with incubation<b><u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u><br><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Multi-well plate imaging<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Incucyte (full course) – phase, green and red fluorescence. System is housed in a standard 37°C incubator with CO2.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><br></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Microscope systems in basement of Loeb open for the MBL community. All are available for the full course.<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><u></u> <u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Zeiss<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Spinning disk confocal (Yokogawa CSUX1) – fully automated with incubation with FRAP and Photoablation from Rapp Opoto.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b><u></u> <u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">880 with Airyscan and FAST – laser scanning confocal with super-res and fast scanning for live cell imaging. Fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">800 – laser scanning confocal fully automated with incubation<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">Widefield system with and injection apparatus.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><b>Nikon<u></u><u></u></b></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">STORM 4 – super-resolution TIRF system. Fully automated and will have incubation for standard TIRF experiments. For Session 2 the system will be equipped with the CU 3D to improve 3D STORM acquisitions.<u></u><u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"><u></u> <u></u></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)"></p><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:Cambria;color:rgb(0,0,0)">A1R+ - resonance scanning confocal system. Fully automated with incubation and autofocus.</p><span class="HOEnZb"><font color="#888888"><div><br></div>-- <br><div data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><table cellpadding="0" cellspacing="0">
<tbody>
<tr>
<td rowspan="3"><b>Steven Wilbert</b><br>Research Assistant<br>Microbiology<br>Forsyth Institute <br><a href="tel:585-802-4733" value="+15858024733" target="_blank">585-802-4733</a> <br></td>
<td><br></td>
<td rowspan="3"><br></td></tr>
<tr>
<td><br></td></tr></tbody></table><br></div></div></div></div>
</font></span></div>
</blockquote></div><br><br clear="all"><div><br></div>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><table cellpadding="0" cellspacing="0">
<tbody>
<tr>
<td rowspan="3"><b>Steven Wilbert</b><br>Research Assistant<br>Microbiology<br>Forsyth Institute <br>585-802-4733 <br></td>
<td><br></td>
<td rowspan="3"><br></td></tr>
<tr>
<td><br></td></tr></tbody></table><br></div></div></div></div>
</div>