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<div>Fully agree with Sally.</div>
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On Jun 11, 2014, at 2:08 PM, "Sally Moody" <<a href="mailto:samoody@gwu.edu">samoody@gwu.edu</a>> wrote:<br>
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<div dir="ltr">Hi Jill,
<div>We don't have that sequence, but in our experience it is better to send it out for sequencing rather than rely on somebody's map because things are not always what they seem. Since it is in pCS, you can use M13 primers or better yet SP6 and T7 primers
for that plasmid.</div>
<div>Good luck, Sally</div>
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<div class="gmail_extra"><br>
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<div class="gmail_quote">On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <span dir="ltr">
<<a href="mailto:johnstoj@ucalgary.ca" target="_blank">johnstoj@ucalgary.ca</a>></span> wrote:<br>
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Hello,<br>
<br>
We have a construct called pCS-GAP43-GFP here and have no information<br>
about it. Does anyone have the sequence that could be sent to us?<br>
<br>
Thanks<br>
<br>
<br>
Jillian Johnston<br>
Research Associate<br>
Dr. Sarah McFarlane lab<br>
University of Calgary<br>
Faculty of Medicine<br>
Dept of Cell Biology and Anatomy<br>
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<div><font face="times new roman,serif">Sally A. Moody, Ph.D.</font></div>
<div><font face="times new roman,serif">Professor </font><font face="times new roman,serif">of Anatomy and Regenerative Biology</font></div>
<div><font face="times new roman,serif">George Washington University</font></div>
<div><font face="Times New Roman">School of Medicine and Health Sciences<br>
2300 I Street, NW, Washington, DC, 20037, USA<br>
202-994-2878<br>
</font></div>
<div><font face="'times new roman', serif">Editor-in-Chief: <i>genesis, The Journal of Genetics and Development</i></font></div>
<div><font face="times new roman, serif">Editor: <i>Principles of Developmental Genetics, 2nd edition</i></font></div>
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