<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="font-family: Times-Roman;">Dear Colleagues,</div><div style="font-family: Times-Roman;">I am currently working on a proposal to the NIH to produce monoclonal antibody libraries to Xenopus proteins. I will use native proteins from embryos and Xenopus cell lines focusing on cell surface and nuclear antigens. </div><div style="font-family: Times-Roman;">1) If you could dream of one antibody that would make your work that much more efficient, which protein would it recognize (Of course one that is not commercial available).</div><div style="font-family: Times-Roman;">2) If anyone has expression data (Proteomics or RNA expression) of XTC, A6 or S3 cell lines that they would be willing to share It could help narrow down which combination to use.</div><div style="font-family: Times-Roman;">The deadline is in 2 weeks.</div><div style="font-family: Times-Roman;">Thanks for your help.</div><div style="font-family: Times-Roman;">Dom </div><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Times; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Times; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="font-size: 14px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">Dominique Alfandari Ph.D.</span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">Associate Professor</span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="font-style: normal; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">Associate Director MCB</span></span></span></span></i></span></span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">Dept. Of Veterinary and Animal Sciences</span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">University of Massachusetts, 661 North Pleasant Street, Amherst MA 01003</span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Times" size="3" color="#0d21ff" style="font: normal normal normal 12px/normal Times; color: rgb(13, 33, 255); font-size: 12px; "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "><span class="Apple-style-span" style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; ">Ph: 413-577-4269 Fax:413-545-6326</span></span></span></span></i></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><i style="font-style: italic; "><span class="Apple-style-span" style="font-style: italic; "><span class="Apple-style-span" style="font-style: italic; "><a href="http://people.umass.edu/alfandar/">http://people.umass.edu/alfandar/</a></span></span></i><font class="Apple-style-span" color="#0D21FF" size="3"><span class="Apple-style-span" style="font-size: 12px; color: rgb(13, 33, 255); "><i style="color: rgb(13, 33, 255); font-size: 12px; font-style: italic; "></i></span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><i><br></i></div><div class="MsoNormal"><font class="Apple-style-span" face="'Brush Script MT'"><div class="MsoNormal"><div class="MsoNormal"><font class="Apple-style-span" face="'Apple Chancery'" size="3"><span class="Apple-style-span" style="font-size: 11px; "><div class="MsoNormal"><span style="font-size: 8pt; font-family: 'Apple Chancery'; ">When the Fathers let the children do anything. When the Sons do not care about their words,<o:p></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt; font-family: 'Apple Chancery'; ">When Teachers tremble in front of their students... When finally the young disregard the laws,<o:p></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt; font-family: 'Apple Chancery'; ">because they do not recognize any longer, above them, the authority from anything or anyone...<o:p></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt; font-family: 'Apple Chancery'; ">Then, in all its beauty and its youth, is the beginning of Tyranny.<o:p></o:p></span></div></span></font></div></div></font></div></span><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Arial" size="3" style="font: normal normal normal 11px/normal Arial; "><span class="Apple-style-span" style="font-family: 'Apple Chancery'; font-weight: bold; ">Platon 428-347 BC.</span></font></div><div><br></div></span></div></span><br class="Apple-interchange-newline"></span><br class="Apple-interchange-newline">
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