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<div>Dear Colleagues,<br>
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We would like to ask for your assistance to promote the 2015 Xenopus Course at Cold Spring Harbor. As many of you know, the Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus
for understanding cell and developmental biology.<br>
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In addition to the traditional skills taught in the Xenopus course, t<font face="ArialMT">his year, we hope to emphasize two approaches: disease gene modeling and biological imaging. We plan to ask students for genes of interest, help them design CRISPR targeting
constructs, and phenotype embryos to simulate human diseases such as congenital malformations.</font><br>
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<font face="ArialMT">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. To this end, we have co-invited
Scott Fraser and will have formal opportunities to interact with QI.</font><br>
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Finally, we hope to tailor the course for 1) students totally new to Xenopus and 2) students already skilled in Xenopus that are interested in starting their own labs or building additional skills.</div>
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<div>We have an exciting list of confirmed speakers/instructors including:<br>
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Helene Cousin<br>
Lance Davidson<br>
Heithem El-Hodiri<br>
Scott Fraser<br>
Rebecca Heald<br>
Ray Keller<br>
Marc Kirschner<br>
Rachel Miller<br>
Nancy Papalopulu<br>
John Wallingford<br>
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A list of topics to be covered is at the end of this email.<br>
Please distribute to any and all students that might be interested. <br>
Applications are due Jan 31, 2015<br>
For more information and application submission, please visit:<br>
<a href="http://meetings.cshl.edu/courses/2015/c-xeno15.shtml">http://meetings.cshl.edu/courses/2015/c-xeno15.shtml</a><br>
<br>
Thanks,<br>
Mustafa Khokha, Yale University<br>
Karen Liu, King’s College, London<br>
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<div style="font-family: ArialMT;">Course objectives:</div>
<div style="font-family: ArialMT;">microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more<br>
in situ hybridization<br>
immunohistochemistry<br>
CRISPR and MO loss of function.<br>
Imaging, microscopy methods, and fluorescence - movies, photography and figure design<br>
transgenesis<br>
Setting up a frog lab - aquatics and microinjection setups/microscopy</div>
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