<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div class="" style="font-family: Helvetica, Arial, sans-serif; font-size: 16px;">
<div class="">
<div class="">
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
Dear Colleagues,<br class="">
<br class="">
Happy holidays to all.</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<br class="">
We are asking for your assistance to promote the 2016 Xenopus Course at Cold Spring Harbor. As many of you know, the Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for
understanding cell and developmental biology.<br class="">
<br class="">
In addition to the traditional skills taught in the Xenopus course, t<span data-mce-style="font-family: ArialMT;" face="ArialMT" class="">his year, we hope to emphasize two approaches: CRISPR based gene modification and biological imaging. We plan to ask students
for genes of interest, help them design CRISPR targeting constructs, and phenotype embryos after CRISPR mediated depletion of the gene product using all of the power of Xenopus. This was a big hit last year, and we hope will continue to be motivating for students
to bring their own projects to the course.</span><br class="">
<br class="">
<span data-mce-style="font-family: ArialMT;" face="ArialMT" class="">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest
imaging methods. This was a huge success last year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods. </span></div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<br class="">
</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<b class="">Important Dates:</b></div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<b class="">Course - April 5-18, 2016</b></div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<b class="">Application Due Date: January 31, 2016</b></div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<br class="">
</div>
<div class=""><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">We have an exciting list of speakers/instructors including:</span><br class="">
<br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Ira Blitz, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University of California,
Irvine</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Sang-Wook Cha, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">Cincinnati Childrens
Hospital Medical Center</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Frank Conlon, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University of North
Carolina</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Helene Cousin,</span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class=""> University of
Massachusetts, Amherst </span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Lance Davidson, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University of
Pittsburgh</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Heithem El-Hodiri,</span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class=""> Nationwide
Childrens Hospital Research Institute </span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Rebecca Heald, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University of
California, Berkeley </span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Raymond Keller, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University of
Virginia </span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Ann Miller</span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">, University of Michigan</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Gerald Thomsen</span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">, Stony Brook University</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">John Wallingford, </span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">University
of Texas at Austin</span><br class="">
<span class="" data-mce-style="font-family: ArialMT; font-weight: bold;" style="font-family: ArialMT; font-weight: bold;">Andrea Wills</span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">, Stanford University</span><br class="">
<br class="">
<span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">For more information and application submission, please visit:</span><br class="">
<span data-mce-style="color: #4787ff; font-family: ArialMT;" face="ArialMT" color="#4787ff" style="color: rgb(71, 135, 255); font-family: ArialMT;" class=""><span data-mce-style="text-decoration: underline;" style="text-decoration: underline;" class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__meetings.cshl.edu_courses.aspx-3Fcourse-3DC-2DXENO-26year-3D16&d=AwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=UJmdFP1E0eiujmyT48xyVLs9SY8woo2_joEgctZcoo8&m=H9Blx52HIeio-u9j0ZHeYBftPXsYjjYnv95XZA6S6UY&s=OqjzjzvVsgqrz4dJJ2H3rLT24g3WoNwPg-eaOkAVwN0&e=" class="" target="_blank">http://meetings.cshl.edu/courses.aspx?course=C-XENO&year=16</a></span></span><br data-mce-bogus="1" class="">
</div>
<div class=""><span data-mce-style="color: #4787ff; font-family: ArialMT;" face="ArialMT" color="#4787ff" style="color: rgb(71, 135, 255); font-family: ArialMT;" class=""><span data-mce-style="text-decoration: underline;" style="text-decoration: underline;" class=""><br class="">
</span></span><span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">Thanks,</span><br class="">
<span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">Mustafa Khokha, Yale University</span><br class="">
<span data-mce-style="font-family: ArialMT;" face="ArialMT" style="font-family: ArialMT;" class="">Karen Liu, King’s College, London</span><br class="">
</div>
</div>
<br class="">
</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
<br class="">
</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
Course objectives:</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more<br class="">
in situ hybridization<br class="">
immunohistochemistry<br class="">
CRISPR and MO loss of function.</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
Germ cell transplants to raise CRISPR lines</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-family: ArialMT;">
Homologous recombination in Xenopus with CRISPR<br class="">
live Imaging and fluorescence - movies, photography and figure design<br class="">
transgenesis<br class="">
</div>
</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-size: 16px; font-family: ArialMT;">
<br class="">
</div>
<div class="" data-mce-style="font-family: ArialMT;" style="font-size: 16px; font-family: ArialMT;">
<br class="">
</div>
<div class="" style="font-family: Helvetica, Arial, sans-serif; font-size: 16px;">
<div class="">
<div class="">Mustafa Khokha</div>
<div class="">Associate Professor</div>
<div class="">Pediatrics & Genetics</div>
<div class="">Yale University School of Medicine</div>
<div class=""><a href="mailto:mustafa.khokha@yale.edu" class="" target="_blank">mustafa.khokha@yale.edu</a></div>
</div>
</div>
</body>
</html>