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<div class="">Dear Colleagues,</div>
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<div class="">In order to encourage applicants to the 2016 Xenopus Course at Cold Spring Harbor, we are extending the application date to Feb. 21st and are able to offer substantial support to offset course costs thanks to support from the NICHD, Helmsley Charitable
Trust, and HHMI to eligible candidates.</div>
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<div class="" style="font-family: ArialMT;">In addition to the traditional skills taught in the Xenopus course, t<span face="ArialMT" class="">his year, we hope to emphasize two approaches: CRISPR based gene modification and biological imaging. We plan to
ask students for genes of interest, help them design CRISPR targeting constructs, and phenotype embryos after CRISPR mediated depletion of the gene product using all of the power of Xenopus. This was a big hit last year, and we hope will continue to be motivating
for students to bring their own projects to the course.</span><br class="">
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<span face="ArialMT" class="">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge
success last year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods. </span></div>
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<div class="" style="font-size: 16px; font-family: ArialMT;"><b class="">Important Dates:</b></div>
<div class="" style="font-size: 16px; font-family: ArialMT;"><b class="">Course - April 5-18, 2016</b></div>
<div class="" style="font-size: 16px; font-family: ArialMT;"><b class="">Application Due Date: February 21st, 2016</b></div>
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<div class="" style="font-size: 16px;"><span face="ArialMT" class="" style="font-family: ArialMT;">We have an exciting list of speakers/instructors including:</span><br class="">
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<span class="" style="font-family: ArialMT; font-weight: bold;">Ira Blitz, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of California, Irvine</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Sang-Wook Cha, </span><span face="ArialMT" class="" style="font-family: ArialMT;">Cincinnati Childrens Hospital Medical Center</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Frank Conlon, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of North Carolina</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Helene Cousin,</span><span face="ArialMT" class="" style="font-family: ArialMT;"> University of Massachusetts, Amherst </span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Lance Davidson, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of Pittsburgh</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Heithem El-Hodiri,</span><span face="ArialMT" class="" style="font-family: ArialMT;"> Nationwide Childrens Hospital Research Institute </span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Rebecca Heald, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of California, Berkeley </span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Raymond Keller, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of Virginia </span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Ann Miller</span><span face="ArialMT" class="" style="font-family: ArialMT;">, University of Michigan</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Gerald Thomsen</span><span face="ArialMT" class="" style="font-family: ArialMT;">, Stony Brook University</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">John Wallingford, </span><span face="ArialMT" class="" style="font-family: ArialMT;">University of Texas at Austin</span><br class="">
<span class="" style="font-family: ArialMT; font-weight: bold;">Andrea Wills</span><span face="ArialMT" class="" style="font-family: ArialMT;">, Stanford University</span><br class="">
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<span face="ArialMT" class="" style="font-family: ArialMT;">For more information and application submission, please visit:</span><br class="">
<span face="ArialMT" class="" style="color: rgb(71, 135, 255); font-family: ArialMT;"><span class="" style="text-decoration: underline;"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__meetings.cshl.edu_courses.aspx-3Fcourse-3DC-2DXENO-26year-3D16&d=AwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=UJmdFP1E0eiujmyT48xyVLs9SY8woo2_joEgctZcoo8&m=H9Blx52HIeio-u9j0ZHeYBftPXsYjjYnv95XZA6S6UY&s=OqjzjzvVsgqrz4dJJ2H3rLT24g3WoNwPg-eaOkAVwN0&e=" class="" target="_blank">http://meetings.cshl.edu/courses.aspx?course=C-XENO&year=16</a></span></span><br class="">
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</span></span><span face="ArialMT" class="" style="font-family: ArialMT;">Thanks,</span><br class="">
<span face="ArialMT" class="" style="font-family: ArialMT;">Mustafa Khokha, Yale University</span><br class="">
<span face="ArialMT" class="" style="font-family: ArialMT;">Karen Liu, King’s College, London</span><br class="">
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<div class="" style="font-size: 16px; font-family: ArialMT;">Course objectives:</div>
<div class="" style="font-size: 16px; font-family: ArialMT;">microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more<br class="">
in situ hybridization<br class="">
immunohistochemistry<br class="">
CRISPR and MO loss of function.</div>
<div class="" style="font-size: 16px; font-family: ArialMT;">Germ cell transplants to raise CRISPR lines</div>
<div class="" style="font-size: 16px; font-family: ArialMT;">Homologous recombination in Xenopus with CRISPR<br class="">
live Imaging and fluorescence - movies, photography and figure design<br class="">
transgenesis<br class="">
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