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--></style></head><body lang="EN-US" link="blue" vlink="purple"><div class="WordSection1"><p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">Hello,</span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif""> </span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">I want to thank everyone for the many responses provided on this survey on mass spectrometry. The feedbacks have been fantastic, and they have helped me greatly to evaluate how mass spectrometry could better research and training in cell/developmental biology. This will be an important component of my proposal. Thank you again for the support.</span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif""> </span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">If you have not yet filled out the form (see link below), the poll is still open until this Friday (July 1). It only takes <2 minutes, and any information you can provide would be greatly appreciated. Your response matters.</span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif""> </span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">With best wishes,</span></p><p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">Peter</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#1f497d"></span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#1f497d"> </span></p><div><p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray">Peter Nemes, PhD</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"></span></p><p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray">Assistant Professor of Chemistry</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"></span></p><p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray">Department of Chemistry, George Washington University, </span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"></span></p><p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray">800 22nd Street, NW, Suite 4000 (Mail)/4880 (Office), Washington, DC 20052</span></p><p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray">202-994-5663, </span><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:#1f497d"><a href="mailto:petern@gwmail.gwu.edu" title="blocked::mailto:petern@gwmail.gwu.edu">petern@email.gwu.edu</a>, <a href="http://home.gwu.edu/~petern" title="blocked::http://home.gwu.edu/~petern">http://home.gwu.edu/~petern</a></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"></span></p></div><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#1f497d"> </span></p><div><div style="border:none;border-top:solid #b5c4df 1.0pt;padding:3.0pt 0in 0in 0in"><p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> <a href="mailto:peter.nemes@gmail.com">peter.nemes@gmail.com</a> [mailto:<a href="mailto:peter.nemes@gmail.com">peter.nemes@gmail.com</a>] <br><b>Sent:</b> Monday, June 27, 2016 12:24 PM<br><b>To:</b> <a href="mailto:petern@email.gwu.edu">petern@email.gwu.edu</a><br><b>Subject:</b> Mass Spectrometry Survey (Dr. Peter Nemes, <a href="mailto:petern@gwu.edu">petern@gwu.edu</a>)</span></p></div></div><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;background:#673ab7"><tr><td style="padding:0in 0in 0in .25in"><p class="MsoNormal"><img border="0" id="_x0000_i1025" src="https://www.gstatic.com/docs/forms/google_forms_logo_lockup_white_2x.png" alt="Google Forms"></p></td></tr></table><div><div style="border:none;border-bottom:solid #e0e0e0 1.0pt;padding:0in 0in 0in 0in;max-width:624px;min-width:154px"><p class="MsoNormal" align="center" style="text-align:center;background:white"><span style="font-family:"Helvetica","sans-serif""> </span></p><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;background:white"><tr><td style="padding:0in 0in 0in 0in"></td></tr><tr><td style="padding:0in 0in 0in 0in"><div><p class="MsoNormal" style="line-height:13.5pt"><b><span style="font-size:10.0pt;color:#424242">I've invited you to fill out a form:</span></b></p></div></td></tr><tr><td style="padding:0in 0in 0in 0in"></td></tr><tr><td style="padding:0in 0in 0in 0in"><p class="MsoNormal" style="line-height:18.0pt"><span style="font-size:15.0pt"><a href="https://docs.google.com/forms/d/1UwvwLosjzr9BHLVb6VSWAXviVv0KUfJlB55et3ATM_M/viewform?c=0&w=1&usp=mail_form_link"><span style="color:#673ab7;text-decoration:none">Mass Spectrometry Survey (Dr. Peter Nemes, petern@gwu.edu)</span></a></span></p></td></tr><tr><td style="padding:0in 0in 0in 0in"></td></tr><tr><td style="padding:0in 0in 0in 0in"><p class="MsoNormal"><span style="font-size:10.0pt;color:#424242">Hello - I am conducting this 2-minute survey as part of a proposal to identify how mass spectrometry (MS) could be better streamlined for biological studies. MS is the technology of choice for measuring known and unknown proteins, peptides, and metabolites in analytical chemistry. For example, my laboratory has recently advanced MS sensitivity to uncover proteomic (Refs. 1, 2) and metabolomic (Refs. 3, 4) cell heterogeneity in early developing embryos of Xenopus laevis. MS offers new, exciting potentials to advance also basic and applied research in the life sciences. <br><br>With your help, I am hopeful to identify where current technical-training limitations are in MS, and how this technology could find mainstream applications also in your research. I am grateful to any suggestions you can offer. Thank you! ~Peter Nemes (George Washington University, Washington, DC)<br><br>References:<br>1. C. Lombard-Banek, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry for discovery proteomics: Quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo, Angew. Chem. Int. Ed. 2016, 55, 2454 –2458, DOI: 10.1002/anie.201510411, PMID 26756663.<br>2. C. Lombard-Banek, Sally A. Moody, and P. Nemes*, Label-free quantification of proteins in single embryonic cells with neural fate in the cleavage-stage frog (Xenopus laevis) embryo using CE-ESI-HRMS, Mol. Cell. Prot. 2016, in press, (Manuscript submission ID: MCP/2015/057760).<br>3. R. M. Onjiko, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo, Proc. Nat. Acad. Sci. USA 2015, 112, 6545–6550, DOI: 10.1073/pnas.1423682112, PMID: 25941375.<br>4. R. M. Onjiko, S. Morris, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry with multi-solvent extraction identifies metabolic differences between left and right blastomeres in the 8-cell frog (Xenopus) embryo, Analyst 2016, 141, 3648–3656, DOI: 10.1039/C6AN00200E <br><br></span></p></td></tr><tr><td style="padding:0in 0in 0in 0in"></td></tr><tr><td style="padding:0in 0in 0in 0in"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%"><tr><td style="padding:0in 0in 0in 0in"><p class="MsoNormal"><a href="https://docs.google.com/forms/d/1UwvwLosjzr9BHLVb6VSWAXviVv0KUfJlB55et3ATM_M/viewform?c=0&w=1&usp=mail_form_link" target="_blank"><b><span style="font-size:10.0pt;color:white;text-transform:uppercase;background:#673ab7;text-decoration:none">FILL OUT FORM</span></b></a></p></td></tr></table></td></tr><tr><td style="padding:0in 0in 0in 0in"></td></tr></table></div></div><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%"><tr><td style="padding:0in 0in 0in 0in"></td></tr><tr><td style="padding:0in 0in 0in 0in"><p class="MsoNormal"><a href="https://docs.google.com/forms?usp=mail_form_link"><span style="font-size:10.0pt;color:#616161">Create your own Google Form</span></a></p></td></tr></table></div><p class="MsoNormal"> </p></div></div></body></html>