<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">My R24 to produce at least 100 monoclonal antibodies to Xenopus protein obtained a very good score likely to be funded sometime in october. This proposal is based on 2 approaches.<div class=""><br class=""><div class="">1 Immunization with Xenopus embryo extracts (Stage 22 membranes and multiple sub fractionation) to produce antibodies that will work on endogenous proteins but without specific protein target. These will be ultimately identified by mass spec. We have produced our first one already against Ribophorin 1 (rpn1) that turns out to be a fantastic loading control and an excellent marker of the TGN by IF.</div><div class="">I fully expect to get some antibodies to Yolk (Vitellogenin) that could be useful to clear ms/ms samples using covalent beads. These will be available as soon as the characterization is done.</div><div class=""><br class=""></div><div class="">2 Immunization with specific proteins that the community request. In order to get that list (I already have a preliminary list). I will need to obtain</div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>1 why is this protein important for the community.</div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>2 a convincing evidence that antibodies against other species do not work (Low conservation)</div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>3 a Flag tagged construct in CS2 or other plasmid with a CMV promotor.</div><div class=""><br class=""></div><div class="">It is much easier to make antibody against cytoplasmic or nuclear proteins so I will likely start with those. I also plan to make antibodies specific to growth factor receptors.</div><div class=""><br class=""></div><div class="">All of these will be distributed via the DSHB as soon as the characterization is complete.</div><div class=""><br class=""></div><div class="">Feel free to contact me about your favorite proteins. I can start before I know if I have the money but I am sure I will have the yolk mAb before that.</div><div class="">Dom</div><div class=""><br class=""><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class=""><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div style="margin: 0px;" class=""><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">Dominique Alfandari Ph.D. </i></font><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">Professor, </i></font></div><div style="margin: 0px;" class=""><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">MCB graduate program director,</i></font></div><div style="margin: 0px;" class=""><i style="color: rgb(13, 33, 255); font-size: 12px;" class="">Dept. Of Veterinary and Animal Sciences</i></div><div style="margin: 0px;" class=""><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">University of Massachusetts, ISB rm 427B. </i></font></div><div style="margin: 0px;" class=""><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">661 North Pleasant Street, Amherst MA 01003</i></font></div><div style="margin: 0px;" class=""><font face="Times" color="#0d21ff" style="color: rgb(13, 33, 255); font-size: 12px;" class=""><i class="">Ph: 413-577-4269 Fax:413-545-6326</i></font></div><div style="margin: 0px;" class=""><i class=""><a href="http://people.umass.edu/alfandar/" class="">http://people.umass.edu/alfandar/</a></i></div><div style="margin: 0px;" class=""><i class=""><br class=""></i></div><div class="MsoNormal"><font class="Apple-style-span" face="'Brush Script MT'"><div class="MsoNormal"><div class="MsoNormal"><font class="Apple-style-span" face="'Apple Chancery'" size="3"><span class="Apple-style-span" style="font-size: 11px;"><div class="MsoNormal"><span style="font-size: 8pt;" class="">When the Fathers let the children do anything. When the Sons do not care about their words,<o:p class=""></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt;" class="">When Teachers tremble in front of their students... When finally the young disregard the laws,<o:p class=""></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt;" class="">because they do not recognize any longer, above them, the authority from anything or anyone...<o:p class=""></o:p></span></div><div class="MsoNormal"><span style="font-size: 8pt;" class="">Then, in all its beauty and its youth, is the beginning of Tyranny.<o:p class=""></o:p></span></div></span></font></div></div></font></div><div style="margin: 0px;" class=""><font face="Arial" style="font-size: 11px; font-family: Arial;" class=""><span class="Apple-style-span" style="font-family: 'Apple Chancery'; font-weight: bold;">Platon 428-347 BC.</span></font></div><div class=""><br class=""></div></span></div></div><br class="Apple-interchange-newline">
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