<html><body><div style="font-family: verdana,helvetica,sans-serif; font-size: 12pt; color: #000000"><div>Dear Xenopus community</div><div> There are several new transgenic lines available for purchase. The names of the lines and their birth dates are listed below:</div><div><br></div><div><div><strong>EB3-GFP (Oct. 2015)</strong></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;">CMV promoter drives expression of GFP in the microtubule.</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">CMV-hEB3-EGFP, consists of a cytomegalovirus (CMV) promoter, 1-281 amino acids of human end-binding protein 3 (hEB3)(Stepanova et al. 2003), and EGFP in pEGFP-N1 (Clontech)</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">Takagi et al. 2013 Dev. Growth Differ 55, 422-433.</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">REMI – Ueno lab</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;"><br></p></div><div><strong>EGFP-Golgi (Oct. 2015)</strong></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;">CMV promoter drives expression of GFP in the Golgi apparatus.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">CMV-hβ4GalT-EGFP, consists of a cytomegalovirus (CMV) promoter, the N terminal 81 amino acids of human β1, 4-galactosyltransferase (β4GalT), and EGFP in pCS2p+.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">Takagi et al. 2013 Dev. Growth Differ 55, 422-433.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">REMI – Ueno lab</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><br></p></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><b>Sox10-GFP (Xla.Tg(-4.7Sox10:GFP))</b><strong style="font-size: 12pt;"> (Oct. 2015)</strong></p></div><div><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">GFP expression in the cranial neural crest cells.</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">Green eyes selection marker.<br> Sox10:GFP-HS4-Isce1 transgene contains 4.7Kb of upstream sequence from the transcription factor sox10 (including part of the 5’ UTR).</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">This is an inert transgene label that reveals the migratory cranial neural crest cell population in early embryos through the distribution of green fluorescent protein.</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">I Sce-I – Saint-Jeannet lab</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;"><br></p></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><strong><span style="font-size: 12.0pt; font-family: 'Times New Roman';" data-mce-style="font-size: 12.0pt; font-family: 'Times New Roman';"><span style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;" data-mce-style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;">RFP-ER (Nov. 2015)</span></span></strong></p><p style="margin: 0px;" data-mce-style="margin: 0px;"><span style="font-size: 12.0pt; font-family: 'Times New Roman';" data-mce-style="font-size: 12.0pt; font-family: 'Times New Roman';"><span style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;" data-mce-style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;"></span></span></p><p style="margin: 0px;" data-mce-style="margin: 0px;">CMV promoter drives expression of RFP in the endoplasmic reticulum. CMV-CALR-RFP-KDEL, consists of a cytomegalovirus (CMV) promoter, the calreticulin (CALR) targeting sequence, RFP, and the KDEL coding sequence in pCS2p+.</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><span style="font-size: 12.0pt; font-family: 'Times New Roman';" data-mce-style="font-size: 12.0pt; font-family: 'Times New Roman';"><span style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;" data-mce-style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;"></span></span></p><p style="margin: 0px;" data-mce-style="margin: 0px;">Takagi et al. 2013 Dev. Growth Differ 55, 422-433.</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><span style="font-size: 12.0pt; font-family: 'Times New Roman';" data-mce-style="font-size: 12.0pt; font-family: 'Times New Roman';"><span style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;" data-mce-style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;"></span></span></p><p style="margin: 0px;" data-mce-style="margin: 0px;">REMI – Ueno lab</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><span style="font-size: 12.0pt; font-family: 'Times New Roman';" data-mce-style="font-size: 12.0pt; font-family: 'Times New Roman';"><span style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;" data-mce-style="font-family: verdana, helvetica, sans-serif; font-size: 12pt;"><br></span></span></p></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><b>mPrx1(Prrx1):GFP (July 2015)</b></p><p style="margin: 0px;" data-mce-style="margin: 0px;">GFP fluorescence in limb bud. The transgene labels mesenchymal cells in limb bud and limb blastema. CMV promoter of pCSGFP3 was removed by SalI and HindIII and a 2.4-kb genomic sequence upstream of mouse prx1 was inserted with the same restriction enzyme sites. Thus, the 2.4-kb mouse prx1 limb-specific enhancer driving GFP3 is inserted into the genome.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">Dev Biol 304 (2007) 675–686</p><p style="margin: 0px;" data-mce-style="margin: 0px;">REMI – Tamura lab</p></div><div><br></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><b>pbin7Lef:dGFP (Feb 2015 and Sept 2015)</b></p><p style="margin: 0px;" data-mce-style="margin: 0px;">GFP expression reveals dynamic patterns of Wnt/β-catenin signalling activity. First clear signals present at neurula stage in the neural folds. The inserted tansgene construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. See Tran et al., 2010 and 2013 for a full description.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">H.T. Tran, K. Vleminckx, Methods (2013),.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">Tran, H., et. al.(2010). Acad. Sci. USA 107 (2010) 16160–16165.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">REMI – Vleminckx lab</p></div><div><br></div><div><br></div><div><strong>Other lines that will be ready to sell in a few months </strong></div><div><br></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><b>RFP-Mem (March 2016)</b></p><p style="margin: 0px;" data-mce-style="margin: 0px;">CMV promoter drives expression of RFP in the plasma cell membrane. CMV-RFP-CAAX, consists of a cytomegalovirus (CMV) promoter, RFP, and the c-Ha-Ras farnesylation signal in pCS2p+.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">Takagi et al. 2013 Dev. Growth Differ 55, 422-433.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">REMI – Ueno lab</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><br></p></div><div><div class="wpb_text_column wpb_content_element "><div class="wpb_wrapper"><p style="margin: 0px;" data-mce-style="margin: 0px;"><span style="font-size: medium;" data-mce-style="font-size: medium;"><b><span style="font-family: Verdana;" data-mce-style="font-family: Verdana;">HyPer (Xla.Tg(crag:RFP, CMV:HyPer-YFP)<sup>Amaya</sup></span></b><span style="font-family: Verdana;" data-mce-style="font-family: Verdana;"><sup> </sup></span></span><strong><span style="text-align: justify; font-size: 12pt;" data-mce-style="text-align: justify; font-size: 12pt;">(March 2016)</span></strong></p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">Ubiquitous hyper YFP expression, marker for integration selection: RFP expression in the eye.</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">g-crys RFP CMV HyPerYFP. (H202 indicator)</p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;">REMI – <span class="Object" id="OBJ_PREFIX_DWT3440_com_zimbra_url"><span class="Object" id="OBJ_PREFIX_DWT3454_com_zimbra_url">Amaya lab</span></span></p><p style="text-align: justify; margin: 0px;" data-mce-style="text-align: justify; margin: 0px;"><span class="Object"><span class="Object"><br></span></span></p></div></div></div></div><div><p style="margin: 0px;" data-mce-style="margin: 0px;"><b>LMO2- Katushka (Xla.Tg(lmo2:Katushka)<sup>EXRC</sup>) (March 2016)</b></p><p style="margin: 0px;" data-mce-style="margin: 0px;">Katushka expression in the blood.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">4775bp X. tropicalis LMO2 promoter driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">X. tropicalis fosmid AOPZ 60793; scaffold 82: 386828-391603.</p><p style="margin: 0px;" data-mce-style="margin: 0px;">I Sce-I – EXRC</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><br></p><p style="margin: 0px;" data-mce-style="margin: 0px;">Feel free to email us if you have questions</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><br></p><p style="margin: 0px;" data-mce-style="margin: 0px;">Sincerely,</p><p style="margin: 0px;" data-mce-style="margin: 0px;">NXR staff</p><p style="margin: 0px;" data-mce-style="margin: 0px;"><br></p></div><div><span name="x"></span><div><div><span style="font-size: small; font-family: arial, helvetica, sans-serif;" data-mce-style="font-size: small; font-family: arial, helvetica, sans-serif;"><strong>****************************************************************</strong></span></div><div><span style="font-size: small; font-family: arial, helvetica, sans-serif;" data-mce-style="font-size: small; font-family: arial, helvetica, sans-serif;"><strong><em>If the NXR contributed to your work please cite us in your papers.</em></strong></span></div><div><br></div><div><span style="font-size: small; font-family: arial, helvetica, sans-serif;" data-mce-style="font-size: small; font-family: arial, helvetica, sans-serif;"><strong><em>Cite us in the acknowledgements: National Xenopus Resource RRID:SCR_013731</em></strong></span></div><div><br></div><div><span style="font-size: small; font-family: arial, helvetica, sans-serif;" data-mce-style="font-size: small; font-family: arial, helvetica, sans-serif;"><strong><em>Cite us in your references: Pearl, E. et al "Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center" Genesis 50, 155-163</em></strong></span></div><div><span style="font-family: arial, helvetica, sans-serif;" data-mce-style="font-family: arial, helvetica, sans-serif;"><span style="font-family: arial, helvetica, sans-serif;" data-mce-style="font-family: arial, helvetica, sans-serif;"><strong><em><br></em></strong></span></span><div><br></div></div><div><span style="font-size: 12pt;" data-mce-style="font-size: 12pt;">-------------------------------------------------------------</span></div>Marko Horb, Ph.D.<br>Director, National Xenopus Resource (NXR)<br>Marine Biological Laboratory<br>7 MBL Street<br>Woods Hole, MA 02543<br></div><div><br></div><div>Email: xenopus@mbl.edu<br>Office: 508-289-7627<br>Cell: 508-564-3764<br></div><div><br></div><div>http://www.mbl.edu/xenopus<br>https://twitter.com/xenopusnxr</div><span name="x"></span><br></div></div></body></html>