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<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><span style="font-family: Arial;">Hello Xine Community,<o:p></o:p></span></p>
<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><span style="font-family: Arial;"> </span></p>
<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><span style="font-family: Arial;">We are writing to inform the community of several new
<i>Xenopus laevis</i> transgenic lines that are now ready for purchase. The lines listed are mature (at least 10-12 months old), unless stated otherwise. If you are interested in any of these lines, please contact<span>
</span></span><a href="mailto:xenopus@mbl.edu" style="color: blue; text-decoration: underline;"><span style="font-family:Arial">xenopus@mbl.edu</span></a><span><span style="font-family: Arial;">.</span></span><span style="font-family: Arial;"><o:p></o:p></span></p>
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<p style="margin-top: 0in; margin-bottom: 6pt;"><b><i><u><span style="font-size: 16pt; font-family: Arial;">X. laevis</span></u></i></b><span style="font-family: Arial;"><o:p></o:p></span></p>
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<!--[if !supportLists]--><span style="font-family: Arial;">1.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(CMV:Hsa.EB3-eGFP)<sup>1Ueno</sup></span></i><span style="font-family: Arial;"> (NXR_0.0109)
<br>
Ubiquitous expression of eGFP in <b>microtubule</b> plus ends.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">2.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial; border: 1pt none windowtext; padding: 0in;">Xla.Tg(CMV:Hsa.B4GALT1-eGFP)<sup>1Ueno</sup>
</span></i><span style="font-family: Arial; border: 1pt none windowtext; padding: 0in;">(NXR_0.110)<br>
Ubiquitous expression of eGFP in the <b>golgi</b>. <br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">3.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(cryga:RFP,CMV:HyPer-YFP)<sup>1Amaya</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0116)<br>
Ubiquitous expression of the <b>hydrogen peroxide indicator</b> HyPer-YFP with <b>
lens</b> expression of RFP as a transgenesis indicator.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><i><span style="font-family: Arial;">4.<span style="font-style: normal; font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span></i><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(CMV:mRFP)<sup>1Ueno</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0115)<br>
Ubiquitous expression of RFP in <b>cell membranes</b>.<br>
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<!--[endif]--><i><o:p></o:p></i></span></p>
<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">5.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(CMV:calr-RFP)<sup>1Ueno</sup></span></i><span style="font-family: Arial;"> (NXR_0.0113)<br>
Ubiquitous expression of RFP in the <b>endoplasmic reticulum</b>.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">6.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(sox10:GFP)<sup>1Jpsj</sup></span></i><span style="font-family: Arial;"> (NXR_0.0108)<br>
GFP expression in the migratory cranial <b>neural crest cells</b>.<br>
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<!--[if !supportLists]--><span style="font-family: Arial;">7.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(Xtr.lmo2:Katushka,cryga:Venus)<sup>1EXRC</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0114)<br>
<b>Blood</b> marked with the red fluorescent protein Katushka, and Venus YFP variant expressed in the
<b>lens</b>.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">8.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(Dre.mpeg1:eGFP);(cryga:mCherry)<sup>1Amaya</sup></span></i><span style="font-family: Arial;"> (NXR_0.0118)<br>
GFP expression in the <b>macrophages</b> and mCherry in the <b>lens</b>.<o:p></o:p></span></p>
<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><span style="font-family: Arial;"> </span></p>
<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><i><span style="font-family: Arial;">The next two transgenic lines are custom orders that the PIs are kindly making available to the wider research community prior to publication. Since they paid for these
frogs, and have first use, we request that individuals wanting these lines contact them before placing an order.<o:p></o:p></span></i></p>
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<!--[if !supportLists]--><span style="font-family: Arial;">1.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(Dre.cdh17:eGFP)<sup>1NXR</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0102)<br>
eGFP expression in the <b>pronephros</b>. Line generated per request from Rachel Miller.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">2.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(tuba1a:deup1-eGFP)<sup>1NXR</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0103)<br>
eGFP expression marking <b>Deuterosome/Cetrioles<i> </i></b>in <b>multi-ciliated cells</b>. Line generated per request from Brian Mitchell.<o:p></o:p></span></p>
<p style="margin-top: 0in; margin-bottom: 0.0001pt;"><span style="font-family: Arial;"> </span></p>
<p style="margin-top: 0in; margin-bottom: 6pt;"><b><u><span style="font-family: Arial;"><br>
</span></u></b></p>
<p style="margin-top: 0in; margin-bottom: 6pt;"><b><u><span style="font-family: Arial;">Juvenile
</span></u></b><b><span style="font-family: Arial;">(these lines are not yet mature and their date of birth is indicated)<u><o:p></o:p></u></span></b></p>
<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">1.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(hsp70:Cre)<sup>1Ryff</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0121) (Dec. 2016)<br>
<b>Heat-shock</b> inducible <b>Cre</b> recombinase.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">2.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(Hsa.UBC:Gal4);(UAS:mapt-Venus);(UAS:mCherry-moe);(cryga:CFP)<sup>1Amaya</sup></span></i><span style="font-family: Arial;"> (NXR_0.0123) (Dec. 2016)<br>
Ubiquitous expression of GFP in <b>microtubules</b> marked by a Tau-GFP fusion, and mCherry in
<b>actin filaments</b> marked by mCherry-moesin fusion. <b>Lens</b> expression of CFP used as a transgenesis reporter.<br>
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<p style="margin-top: 0in; margin-left: 0.5in; margin-bottom: 0.0001pt; text-indent: -0.25in;">
<!--[if !supportLists]--><span style="font-family: Arial;">3.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(rho:eGFP-ATP1a3)<sup>1Baker</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0122) (Nov. 2016)<br>
Xenopus <i>rhodopsin </i>promoter driving expression of eGFP tagged neuronal <i>á</i>3 isoform of the Na<sup>+</sup>/K<sup>+</sup>-ATPase in the
<b>rod photoreceptors</b>.<br>
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<p style="margin-left:.5in;text-indent:-.25in;mso-outline-level:1;mso-list:
l0 level1 lfo3">
<!--[if !supportLists]--><span style="font-family: Arial;">4.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(tubb2b:ChIEF,Rno.elas:GFP)<sup>1NXR</sup>
</span></i><span style="font-family: Arial;">(NXR_0.0125) (Feb. 2017)<br>
<b>Neural</b> expression of blue light <b>photoinducible</b> channelrhodopsin, ChIEF, and expression of GFP in the
<b>pancreas</b>.<br>
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<p style="margin-left:.5in;text-indent:-.25in;mso-outline-level:1;mso-list:
l0 level1 lfo3">
<!--[if !supportLists]--><span style="font-family: Arial;">5.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";">
</span></span><!--[endif]--><i><span style="font-family: Arial;">Xla.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)<sup>1NXR</sup></span></i><span style="font-family: Arial;"> (NXR_0.0107): (May 2017)<br>
<b>Neural</b> expression of the green <b>Ca<sup>2+</sup> sensor</b>, GCaMP6s, and expression of GFP in the
<b>pancreas</b>.<o:p></o:p></span></p>
<br>
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<span style="font-family:Georgia,serif"></span><span class="Object" id="OBJ_PREFIX_DWT17957_com_zimbra_email" style="color:rgb(51,102,153); font-family:arial,helvetica,sans-serif; font-size:16px; background-color:rgb(252,253,253)"><span class="Object" id="OBJ_PREFIX_DWT18045_com_zimbra_email" style=""><a href="mailto:smcnamara@mbl.edu" target="_blank" style="color:rgb(51,102,153)" id="LPNoLP"><span style="font-family:Georgia,serif">smcnamara@mbl.edu</span></a></span></span><br style="font-family:arial,helvetica,sans-serif; font-size:16px; background-color:rgb(252,253,253)">
<span style="font-family:Georgia,serif"></span><span style="font-family:Georgia,serif; font-size:16px; background-color:rgb(252,253,253)">Lab:</span><span style="font-family:Georgia,serif; font-size:16px; background-color:rgb(252,253,253)"> </span><span class="Object" id="OBJ_PREFIX_DWT17958_com_zimbra_phone" style="color:rgb(51,102,153); font-family:arial,helvetica,sans-serif; font-size:16px; background-color:rgb(252,253,253)"><a href="callto:508 289 7125" style="color:rgb(51,102,153)" id="LPNoLP"><span style="font-family:Georgia,serif">508
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