<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Dear all,<div class=""><br class=""></div><div class="">I am searching for the X. laevis mutant that, as the heterozygote 1-<i class="">nu</i>, lacks one nucleolus in most of their diploid cells. This mutant was described by Elsdale et al. (1) and was then further characterized by Brown & Gurdon (2, 3). Any information on live animals would be appreciated.</div><div class=""><br class=""></div><div class="">Thanks and kind regards</div><div class="">Reinhard Obst</div><div class=""><br class=""></div><div class="">1<span class="Apple-tab-span" style="white-space:pre"> </span>Elsdale TR, Fischberg M, Smith S. (1958) A mutation that reduces nucleolar number in Xenopus laevis. Exp Cell Res. 14: 642-3. PMID: 13562098.</div><div class="">2<span class="Apple-tab-span" style="white-space:pre"> </span>Brown DD, Gurdon JB. (1964) Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. PNAS 51: 139-46. PMID: 14106673.</div><div class="">3<span class="Apple-tab-span" style="white-space:pre"> </span>Brown DD, Gurdon JB. (1966) Size distribution and stability of DNA-like RNA synthesized during development of anucleolate embryos of Xenopus laevis. J Mol Biol 19: 399-422. PMID: 5969074.</div><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; widows: 2; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">.............................................................................<br class=""></div></span></span></div></span></div></span></div></span></div></span></div></span><span class="Apple-style-span" style="orphans: 2; widows: 2; color: rgb(44, 104, 7); font-size: 11px;"><font class="Apple-style-span" face="Tahoma"><b class="">LMU</b></font></span><span class="Apple-style-span" style="orphans: 2; widows: 2; border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"> </span><span class="Apple-style-span" style="text-align: -webkit-auto; orphans: 2; widows: 2; border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;">Ludwig-Maximilians-Universität München<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; text-align: -webkit-auto; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class="">Institute for Immunology<br class="">PD Dr. Reinhard Obst</div><div class="">Biomedical Center<br class=""><div class="">Großhaderner Strasse 9</div><div class="">82152 Planegg-Martinsried</div><br class=""></div><div class="">Email<span class="Apple-tab-span" style="white-space: pre;"> </span><a href="mailto:reinhard.obst@med.uni-muenchen.de" class="">reinhard.obst@med.uni-muenchen.de</a></div><div class=""><span style="text-align: -webkit-auto;" class="">Tel</span><span class="Apple-tab-span" style="text-align: -webkit-auto; white-space: pre;"> </span><span style="text-align: -webkit-auto;" class="">+49 89 2180 75631</span></div><div class="">Fax<span class="Apple-tab-span" style="white-space: pre;"> </span>+49 89 2180 99 75631</div><div class=""><br class=""></div><div class=""><a href="http://www.immunologie.med.uni-muenchen.de" class="">www.immunologie.med.uni-muenchen.de</a><br class="">.............................................................................</div><div class=""><br class=""></div></div></span></span></div></span></div></span></div></span></div></span></div></span></div><div class=""><br class=""></div></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
</div>
<br class=""></body></html>