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We do not have the xenopus sequences for expression in PCS2. We have been expressing the mouse sequences. We could send the longest xenopus clones of each that we have.
<div class="">I think I said this before, but HCN4 and HCN3 were mis-identified in Xenopus as well as others species. We have the correct Hcn4 as in in situ probe.</div>
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<div class="">Joan</div>
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<div class="">On Sep 18, 2017, at 6:21 AM, John Gurdon <<a href="mailto:j.gurdon@gurdon.cam.ac.uk" class="">j.gurdon@gurdon.cam.ac.uk</a>> wrote:</div>
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Dear Genevieve et al.
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<div class=""><span class="Apple-tab-span" style="white-space:pre"></span>Dr Gurdon would be most interested to see this list of antibodies you have against transcription factors if you have this information.</div>
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<div class="">Yours sincerely,</div>
<div class="">Dilly Bradford</div>
<div class="">Secretary/PA</div>
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<div class="">On 8 Sep 2017, at 15:59, Almouzni Pettinotti Genevieve wrote:</div>
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<div class="">Super !!!</div>
<div class="">Bravo</div>
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Envoyé de mon iPhone</div>
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Le 8 sept. 2017 à 16:52, Dominique Alfandari <<a href="mailto:alfandar@vasci.umass.edu" class="">alfandar@vasci.umass.edu</a>> a écrit :<br class="">
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<div class="">Dear colleagues,
<div class="">I am happy to say that we have finally received the notice of award for our NIH grant to produce and characterize 100 monoclonal antibody against Xenopus proteins. </div>
<div class="">This is a 4 year grant that is designed to produce antibodies to membrane protein, signaling receptors and transcription factors using multiple approaches. The essential goal of our project is to produce antibodies that work in embryos by blot/IF/IP.</div>
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<div class="">One part of the project is to identify key proteins, for which there is no cross reacting antibodies, and that are critical for the community. These targets will be produced as flag-tagged protein expressed in Hek293T cells and used to immunize
mice. </div>
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<div class="">For people working on transcription factors, I would strongly recommend that you look at the antibodies developed by the DSHB. They provide sup for very cheap. We have been able to test several of them that do cross react (e.g Arid3a, Sox10).</div>
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<div class="">If you are interested and would like (1 to propose a target or (2 be on a committee that help select the highest priority targets, please email me. (Some of you have done this a long time ago but a refresher could be good).</div>
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<div class="">1 what is your target?</div>
<div class="">2 What did you do to find if existing antibody may cross react?</div>
<div class="">3 Do you have your full length tagged in CS2 with Flag (any other vector with a CMV promoter is acceptable).</div>
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<div class="">All antibody will be validated on embryo extract/whole mount immunostaining/IP-LC/ms/ms and will be available at the DSHB. The characterization files will be integrated in Xenbase with the appropriate gene page.</div>
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<div class="">I plan to hire 2 technicians for this project (Add to follow).</div>
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<div class="">Thank you for your attention. I hope to hear from you.</div>
<div class="">Dom</div>
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<div class=""><b class=""><i class="">Dominique Alfandari</i></b></div>
<div class=""><b class=""><i class="">PhD. Professor </i></b></div>
<div class=""><b class=""><i class=""><a href="mailto:alfandar@vasci.umass.edu" class="">alfandar@vasci.umass.edu</a></i></b></div>
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<div class="">Dr John Gurdon</div>
<div class="">The Gurdon Institute, University of Cambridge,</div>
<div class="">Tennis Court Road, Cambridge CB2 1QN, United Kingdom</div>
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<div class="">Tel: +44 (0)1223 334090 Fax: +44 (0)1223 334089</div>
<div class="">Email: <a href="mailto:j.gurdon@gurdon.cam.ac.uk" class="">j.gurdon@gurdon.cam.ac.uk</a></div>
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