<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Dear all, the first mice have been immunized with membrane extract (stage 24) and the first set of transcription factor Six1, Slug, Sox3, Twist and FoxD3.<div class="">I am missing a lot of plasmid for our top choices.</div><div class="">If you have expression constructs (Flag is best but myc can work too) for the following plasmids, please send them to me ASAP. I do not currently have the capacity to clone all of the targets.</div><div class="">
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<td height="15" class="xl65" width="65" style="height:15.0pt;width:65pt">Ago
(ago1/2/3/4)</td>
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<td height="15" class="xl68" style="height:15.0pt">Sox8</td>
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<td height="15" class="xl65" style="height:15.0pt">Ascl1</td>
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<td height="15" class="xl65" style="height:15.0pt">Neurog2</td>
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<td height="15" class="xl65" style="height:15.0pt">Smad7</td>
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<td height="15" class="xl65" style="height:15.0pt">ADMP</td>
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<td height="15" class="xl65" style="height:15.0pt">Ets1</td>
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<td height="15" class="xl65" style="height:15.0pt">Eya1</td>
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<td height="15" class="xl65" style="height:15.0pt">Gbx2</td>
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<td height="15" class="xl65" style="height:15.0pt">Hcn3 ( or hcn2)</td>
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<td height="15" class="xl65" style="height:15.0pt">Hcn4</td>
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<td height="15" class="xl65" style="height:15.0pt">HoxD1</td>
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<td height="15" class="xl65" style="height:15.0pt">Neurogenin3 (neurog3)</td>
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<td height="15" class="xl65" style="height:15.0pt">Notch1ICD</td>
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<td height="15" class="xl65" style="height:15.0pt">Par3 (pard3)</td>
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<td height="15" style="height:15.0pt" class="">Rax</td>
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<td height="15" style="height:15.0pt" class="">ROCK</td>
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<td height="15" style="height:15.0pt" class="">Shroom3<br class=""><br class=""></td>
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<div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); font-family: Times; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div><b class=""><i class="">Dominique Alfandari</i></b></div><div><b class=""><i class="">PhD. Professor </i></b></div><div><b class=""><i class=""><a href="mailto:alfandar@vasci.umass.edu" class="">alfandar@vasci.umass.edu</a></i></b></div><div><div class="">661 north pleasant street,</div><div class="">ISB rm 427B, Amherst, MA, 01003</div><div class=""><br class=""></div></div></div></div></div></div><br class="Apple-interchange-newline">
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