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Dear Colleagues,<br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font>Happy New Year to all.<br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font>We are asking for your assistance to promote the 2018 Xenopus Course at Cold Spring Harbor. The Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for understanding
cell and developmental biology.<br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font>In order for students to get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest, we will design sgRNAs for these genes, and then students can knock these genes out at the
course and analyze any phenotypes using all of the tools available from advanced high-speed fluorescent confocal imaging to cut-and-paste embryology.<br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font><span style="font-family: ArialMT;" class="">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods.
This was a huge success last year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods. </span><br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font><b class="">Important Dates:</b><br class="">
<b class="">Course - April 4-17, 2018</b><br class="">
<b class="">Application Due Date: January 31, 2018</b><br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font><span style="font-family: ArialMT;" class="">We have an exciting list of speakers/instructors including:</span><br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font><span class="" style="font-weight: bold;">Ira Blitz, </span><font face="ArialMT" class="">University of California, Irvine</font><br class="">
<span class="" style="font-weight: bold;">Sang-Wook Cha, </span><font face="ArialMT" class="">Cincinnati Childrens Hospital Medical Center</font><br class="">
<span class="" style="font-weight: bold;">Frank Conlon, </span><font face="ArialMT" class="">University of North Carolina</font><br class="">
<span class="" style="font-weight: bold;">Chenbei Chang,</span><font face="ArialMT" class=""> University of Alabama</font><br class="">
<span class="" style="font-weight: bold;">Lance Davidson, </span><font face="ArialMT" class="">University of Pittsburgh</font><br class="">
<span class="" style="font-weight: bold;">Roberto Mayor,</span><font face="ArialMT" class=""> University College London</font><br class="">
<span class="" style="font-weight: bold;">Rebecca Heald, </span><font face="ArialMT" class="">University of California, Berkeley </font><br class="">
<span class="" style="font-weight: bold;">Raymond Keller, </span><font face="ArialMT" class="">University of Virginia </font><br class="">
<span class="" style="font-weight: bold;">Bill Bement</span><font face="ArialMT" class="">, University of Wisconsin-Madison</font><br class="">
<span class="" style="font-weight: bold;">Gerald Thomsen</span><font face="ArialMT" class="">, Stony Brook University</font><br class="">
<span class="" style="font-weight: bold;">John Wallingford, </span><font face="ArialMT" class="">University of Texas at Austin</font><br class="">
<span class="" style="font-weight: bold;">Andrea Wills</span><font face="ArialMT" class="">, University of Washington</font><br class="">
<b class="">Frank Conlon</b>, University of North Carolina<br class="">
<b class="">Peter Nemes</b>, University of Maryland<br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
</font><span style="font-family: ArialMT;" class="">For more information and application submission, please visit:</span><br class="">
<u class="" style="color: rgb(71, 135, 255); font-family: ArialMT;"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__emea01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fmeetings.cshl.edu-252Fcourses.aspx-253Fcourse-253DC-2DXENO-2526year-253D18-26data-3D01-257C01-257Ckaren.liu-2540kcl.ac.uk-257C4e1f96db16e44cfd54a208d551e40747-257C8370cf1416f34c16b83c724071654356-257C0-26sdata-3DdlDc829-252Fzf3tTAy-252BKzuuBehkXi5gGVELgADBDZuL1os-253D-26reserved-3D0&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LA_1WRYwu9TpwOjhkdSG-AujoUacW8JYAUaWHgVP3Bg&m=SUseUAjeroKJ_TebWbybNGMQ-wiA98v-Ghtf-n6SlLU&s=kCEabIBXhR8ViCuR7hP_MUPeBEgrVn5-EQPA1N58PH8&e=" class="">http://meetings.cshl.edu/courses.aspx?course=C-XENO&year=18</a></u><br class="">
<font color="#4787ff" face="ArialMT" class=""><u class=""><br class="">
</u></font><span style="font-family: ArialMT;" class="">Thanks,</span><br class="">
<span style="font-family: ArialMT;" class="">Mustafa Khokha, Yale University</span><br class="">
<span style="font-family: ArialMT;" class="">Karen Liu, King’s College, London</span><br class="">
<font color="#5856d6" face="ArialMT" class=""><br class="">
<br class="">
</font>Course objectives:<br class="">
microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more<br class="">
in situ hybridization<br class="">
immunohistochemistry<br class="">
CRISPR and MO loss of function.<br class="">
Germ cell transplants to raise CRISPR lines<br class="">
Homologous recombination in Xenopus with CRISPR<br class="">
live Imaging and fluorescence - movies, photography and figure design<br class="">
transgenesis<br class="">
Proteomics approaches<br class="">
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<div class="">Regeneration</div>
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