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<p class="MsoNormal">Dear Xenopus community<o:p></o:p></p>
<p class="MsoNormal"> I am pleased to announce we have two new centriole transgenic lines that were generated for Brian Mitchell and which he has kindly made available to the community prior to publication. These two lines are:<o:p></o:p></p>
<p class="MsoNormal"><b>Xla.Tg(tuba1a:deup1-eGFP)</b><sup>NXR</sup><o:p></o:p></p>
<p class="MsoNormal"><b> </b><b><span style="font-size:11.0pt;color:black"> </span></b><span style="font-size:11.0pt;color:black">Tubulin promoter<b> </b>driving<b> </b>expression of Deup1 fused to eGFP labeling both deuterosomes and centrioles specifically
in multi-ciliated cells. This is a great marker for visualizing centrioles. </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><b><o:p> </o:p></b></p>
<p class="MsoNormal"><b>Xla.Tg(CMV:cetn4-RFP)</b><sup>NXR</sup><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;color:black"> CMV promoter driving expression of centrin 4 (cetn4) fused with RFP labeling centrioles in all cells. Note: while the expression level and centriole fluorescent intensity is quite
high in most cells, the number of centrioles in multi-ciliated cells appears to dilute the intensity/centriole. </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Sincerely,<o:p></o:p></p>
<p class="MsoNormal">Marko Horb<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:black">**************************************************************************</span><span style="font-size:10.5pt;color:black"><br>
</span><b><i><span style="color:black">If the NXR contributed to your work please cite us in your papers.</span></i></b><span style="font-size:10.5pt;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><i><span style="color:black">Cite us in Materials and Methods as National Xenopus Resource RRID:SCR_013731 at the Marine Biological Laboratory</span></i></b><span style="font-size:10.5pt;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><i><span style="color:black">Cite us in your references: Pearl, E. et al "Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center" Genesis 50, 155-163</span></i></b><span style="font-size:10.5pt;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">---------------------------------------------------------------------------------------<br>
Marko Horb, Ph.D. |Director, National <i>Xenopus</i> Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering<br>
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">Email:
</span><span lang="FR" style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"><a href="mailto:mhorb@mbl.edu"><span lang="EN-US" style="color:#0563C1">mhorb@mbl.edu</span></a></span><span lang="FR" style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">
</span><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">| Office: 508-289-7627 | Lab: 508-289-7370 | Cell: 508-564-3764<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">http://www.mbl.edu/horb<br>
http://www.mbl.edu/xenopus<br>
https://twitter.com/xenopusnxr<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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