<div dir="ltr">Fantastic work, Dom. our efforts are much appreciated.</div><div class="gmail_extra"><br clear="all"><div><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div><font face="times new roman,serif">Sally A. Moody, Ph.D., FAAA</font></div>
<div><font face="times new roman,serif">Professor and Chair </font><font face="times new roman,serif">of Anatomy and Cell Biology</font></div>
<div><font face="times new roman,serif">George Washington University</font></div>
<div><font face="Times New Roman">School of Medicine and Health Sciences<br>2300 I Street, NW, Washington, DC, 20037, USA<br>202-994-2878</font></div><div>Fellow of the American Association of Anatomists</div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>
<br><div class="gmail_quote">On Thu, Oct 11, 2018 at 9:06 AM, Dominique Alfandari <span dir="ltr"><<a href="mailto:alfandar@vasci.umass.edu" target="_blank">alfandar@vasci.umass.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space">Dear Colleagues, <div>we have now been working to make monoclonal antibody to Xenopus proteins for one year. I wanted to update you on what we have and what we are doing now.</div><div>We have screened by immunoprecipitation more than 400 clones from mice immunized with cytoplasmic and membrane proteins isolated from tailbud stage Xenopus embryos. We have identified more than 50 antibodies that efficiently IP proteins of different sizes. We are grouping them in Size groups and will start the final characterization by mass spec in the coming month. We have about 1000 more to screen.</div><div><br></div><div>As for targets:</div><div>We have mAb to Ror2, Par3, Rax1, Lbh, PCNS</div><div>We have splenocytes for Xbra, Smad7, Sox3, Ror2, Rax</div><div>We have mice immunized with Ngn2, Slug, Sox8, Twist, Prdm12, Sox9, FoxD3, NeuroD.</div><div><br></div><div>The antibody all work by IP and IF on transfected cells, most work by IP on embryo extracts. Some are being characterized in labs that originally requested them. As soon as we are satisfied with the characterization we will make them available and write a descriptive sheet for all of them. If you want some sup to test yourself, contact me with you FEDEX number.</div><div><br></div><div>We just invested in an electrofusion system which appears to be magnitudes more efficient than our traditional PEG in our first experiment. From Frozen splenocites, we obtain 7 fold greater number of hybridoma than with fresh spleen with PEG. The proportion of hybridoma producing IgG to the targets is also much greater.</div><div><br></div><div>Dom<span class="HOEnZb"><font color="#888888"><br><div>
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word"><div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word"><div style="word-wrap:break-word"><div style="color:rgb(0,0,0);font-family:Times;font-size:14px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px"><div><b><i>Dominique Alfandari</i></b></div><div><b><i>PhD. Professor </i></b></div><div><b><i><a href="mailto:alfandar@vasci.umass.edu" target="_blank">alfandar@vasci.umass.edu</a></i></b></div><div><div>661 north pleasant street,</div><div>ISB rm 427B, Amherst, MA, 01003</div><div><br></div></div></div></div></div></div><br class="m_7847363923893244886Apple-interchange-newline">
</div>
<br></font></span></div></div><br>______________________________<wbr>_________________<br>
Xenopus mailing list<br>
<a href="mailto:Xenopus@lists.mbl.edu">Xenopus@lists.mbl.edu</a><br>
<a href="https://lists.mbl.edu/mailman/listinfo/xenopus" rel="noreferrer" target="_blank">https://lists.mbl.edu/mailman/<wbr>listinfo/xenopus</a><br>
<br></blockquote></div><br></div>