<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Dear colleagues, <div class="">I am looking for technicien, graduate student and postdoc. The two main project in my lab are:</div><div class="">1 Understanding cranial neural crest cell migration.</div><div class="">2 Production of monoclonal antibody to Xenopus proteins.</div><div class=""><br class=""></div><div class="">Project number 1 is for people interested in academic careers while project 2 is a great training opportunity for industry jobs.</div><div class="">Thank you for spreading the word.</div><div class=""><br class=""></div><div class="">On another note, the antibody project is progressing well. I will start uploading available antibody with description on Xenbase soon. In the mean time we have good antibodies to the following proteins. Complete characterization is still in the way but all work by either western blot or immunofluorescence on the target protein express in XTC cells, many of them also work on the endogenous proteins in the embryo.</div><div class="">Antigen list is as follow:</div><div class="">
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<!--StartFragment--><p class="MsoNormal"><b class="">Mab List as of
12-17-18.<o:p class=""></o:p></b></p><p class="MsoNormal"><b class="">LBH -1<o:p class=""></o:p></b></p><p class="MsoNormal">1 DA2B8lbh, WB, IP, Fluo, Trimaway, endogenous<o:p class=""></o:p></p><p class="MsoNormal"><b class="">PCNS -1<o:p class=""></o:p></b></p><p class="MsoNormal">2 DA2F4pcns, WB, IP, Fluo, endogenous<o:p class=""></o:p></p><p class="MsoNormal"><b class="">Par3 -6<o:p class=""></o:p></b></p><p class="MsoNormal">3 DA8B4par3, WB, IP, IF, endogenous<o:p class=""></o:p></p><p class="MsoNormal"><b class=""><span style="color:red" class="">Rax1 -1 (Frozen splenocyte for additional fusion)<o:p class=""></o:p></span></b></p><p class="MsoNormal">4 DA2F6rax1, WB, IF<o:p class=""></o:p></p><p class="MsoNormal"><b class="">Sox3 -4<o:p class=""></o:p></b></p><p class="MsoNormal">5 DA2C5sox3, WB, IF, endogenous<o:p class=""></o:p></p><p class="MsoNormal"><b class=""><span style="color:red" class="">FoxD3 -1<o:p class=""></o:p></span></b></p><p class="MsoNormal">6 DA3F10foxD3, WB, IF, endogenous (Cross reaction)<o:p class=""></o:p></p><p class="MsoNormal"><b class="">Ror2 -17<o:p class=""></o:p></b></p><p class="MsoNormal">7 DA12B3ror2, WB endogenous<o:p class=""></o:p></p><p class="MsoNormal">8 DA12H4ror2, WB endogenous<o:p class=""></o:p></p><p class="MsoNormal">9 DA6D11ror2, IF endogenous<o:p class=""></o:p></p><p class="MsoNormal">10 DA9D10ror2, IF endogenous<o:p class=""></o:p></p><p class="MsoNormal"><b class=""><span style="color:red" class="">Ngn2 -10 (Frozen splenocyte for additional fusion)<o:p class=""></o:p></span></b></p><p class="MsoNormal">11 DA9H1ngn2, WB, IF<o:p class=""></o:p></p><p class="MsoNormal"><b class="">Sox8 -2<o:p class=""></o:p></b></p><p class="MsoNormal">12 DA11B7sox8, IF<o:p class=""></o:p></p><p class="MsoNormal"><o:p class=""> </o:p></p>
<!--EndFragment--><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); font-family: Times; font-size: 14px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div><b class=""><i class="">Dominique Alfandari</i></b></div><div><b class=""><i class="">PhD. Professor </i></b></div><div><b class=""><i class=""><a href="mailto:alfandar@vasci.umass.edu" class="">alfandar@vasci.umass.edu</a></i></b></div><div><div class="">661 north pleasant street,</div><div class="">ISB rm 427B, Amherst, MA, 01003</div><div class=""><br class=""></div></div></div></div></div></div><br class="Apple-interchange-newline">
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