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<p class="MsoNormal">Dear Colleagues,<span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Greetings and salutations to all.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We are asking for your assistance to promote the 2020 Xenopus Course at Cold Spring Harbor. The Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for
understanding cell and developmental biology. <b>Stipends are available to offset tuition costs (see the website for details).</b><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest, we will design sgRNAs for these genes, and then students can knock these genes out at the course
and analyze any phenotypes using all of the tools available from advanced high-speed fluorescent confocal imaging to cut-and-paste embryology.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success last
year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Important Dates:<o:p></o:p></p>
<p class="MsoNormal">Course – March 25-April 7, 2020<o:p></o:p></p>
<p class="MsoNormal"><b>Application Due Date: January 31, 2020<o:p></o:p></b></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have an exciting list of speakers/instructors including:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Chenbei Chang, University of Alabama;<o:p></o:p></p>
<p class="MsoNormal">Lance Davidson, University of Pittsburgh;<o:p></o:p></p>
<p class="MsoNormal">Rebecca Heald, University of California at Berkeley;<o:p></o:p></p>
<p class="MsoNormal">Douglas Houston, University of Iowa;<o:p></o:p></p>
<p class="MsoNormal">Mustafa Khokha, Yale University;<o:p></o:p></p>
<p class="MsoNormal">Ray Keller, University of Virginia;<o:p></o:p></p>
<p class="MsoNormal">Roberto Mayor, University College London, UK;<o:p></o:p></p>
<p class="MsoNormal">Bryan Mitchell, Northwestern University;<o:p></o:p></p>
<p class="MsoNormal">Nanette Nascone-Yoder, North Carolina State University;<o:p></o:p></p>
<p class="MsoNormal">Andrea Wills, University of Washington;<o:p></o:p></p>
<p class="MsoNormal">Sarah Woolner, University of Manchester, UK;<o:p></o:p></p>
<p class="MsoNormal">Martin Wuhr, Princeton University<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">For more information and application submission, please visit the
<span class="MsoHyperlink"><a href="http://meetings.cshl.edu/courses.aspx?course=C-XENO&year=19">website</a></span>.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks,<o:p></o:p></p>
<p class="MsoNormal">Chenbei Chang, University of Alabama<o:p></o:p></p>
<p class="MsoNormal">Lance Davidson, University of Pittsburgh<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Course objectives:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more. In situ hybridization, immunohistochemistry, CRISPR, and MO loss of function.<o:p></o:p></p>
<p class="MsoNormal">Germ cell isolation and transplants. Live cell and embryo Imaging and fluorescence - confocal microscopy, image analysis and figure design. Genomic and proteomic approaches to development, regeneration, and disease models.<o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
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