<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Times New Roman \(Body CS\)";
panose-1:2 11 6 4 2 2 2 2 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
span.apple-converted-space
{mso-style-name:apple-converted-space;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style>
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E">Dear Xenopus Community,</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> </span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> I am happy to announce that we have just been awarded a grant from the NIH (ORIP), which provides funding to create a new “Xenopus Mutant Resource”. The goal of this resource is to promote the use of<span class="apple-converted-space"> </span><i>Xenopus<span class="apple-converted-space"> </span></i>in
modeling human disease mutants. First, it provides funding to continue generating novel mutants here at the NXR. Second, it provides funding to make visits to the NXR more affordable for researches interested in working with our mutant and transgenic animals.
Third, it supports the creation of new transgenic animals for genome editing. If you have any specific lines you want made please contact us. Fourth, we will also be working on creating new knockin lines, so once we have that going well we will request from
you what knockins you would want.</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> </span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> My plan is to have 2 new research assistants working on this project (in addition to the 3 RAs currently in the lab), making at least 25 new mutant lines per year. If you would like a specific mutant
made, email <a href="mailto:xenopus@mbl.edu" target="_blank"><span style="font-size:11.0pt;border:none windowtext 1.0pt;padding:0in">xenopus@mbl.edu</span></a> and we can start the process. My RAs will work closely with you to make the mutant in the gene you
want and the region you want. Furthermore, the touristy nature of Woods Hole results in high demand and relatively high cost of short term housing, especially during the summer months.<span class="apple-converted-space"> </span> With the funding provided by
this grant I have rented one of the cottages here at the MBL and it is available at no cost to the researchers wanting to visit the NXR. It can accommodate up to 6 individuals and my hope is that people take advantage of this throughout the year (and not just
the summer). Since the summer will be in high demand in the future, we may need to set some rules to accommodate everyone; but that will be a good problem to have. You will still need to pay for your travel and there is a small charge of $500/week to cover
consumables that goes back to the NXR as part of our Program Income, but you do get access to all of our animal lines, our lab that is equipped with everything you will need, and an RA assigned to help you with your project. In particular, you will have access
to more than one hundred mutant lines we have already created over the past several years. Many of the lines are sexually mature F1 heterozygous animals, with the mutant sequence characterized by us. They simply require phenotypic analysis of the F2 homozygous
mutant embryos and therefor have a high potential for rapidly generating mutant data We are working on developing a system for reservations, but for now please email </span><span style="color:#201F1E;border:none windowtext 1.0pt;padding:0in"><a href="mailto:nxrorders@mbl.edu">nxrorders@mbl.edu</a></span><span style="font-size:12.0pt;color:#201F1E"> to
request information and to reserve your time in the cottage. My hope is that this allows people to coordinate their visits, and of course share the cottage to develop new collaborations.<o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E"><o:p> </o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E"> I am also hoping to hire a postdoc to work on advancing CRISPR in Xenopus with this grant. If you know of anyone who would be interested please
have them contact me.</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> <span class="apple-converted-space"> </span></span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;background-position:initial initial;background-repeat:initial initial;word-spacing:0px">
<span style="font-size:12.0pt;color:#201F1E"> Finally, I’d like to thank both ORIP at the NIH who funded the grant this year and the<span class="apple-converted-space"> </span><i>Xenopus</i><span class="apple-converted-space"> </span>community as
without your support this would not be possible. I’d like to stress that both this grant and the resources of the NXR are here to serve the community. Please take advantage of them, now that getting custom mutants made and visiting the NXR is even more affordable.
We look forward to helping you advance your research. I will follow up with another email once we are able to host scientists here at the MBL. Thank you all for your support and I do hope you take advantage of this resource.<o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E"><o:p> </o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E">Sincerely,<o:p></o:p></span></p>
<p style="margin:0in;margin-bottom:.0001pt;background:white"><span style="font-size:12.0pt;color:#201F1E">The XMR team</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:black">**************************************************************************</span><span style="font-size:10.5pt;color:black"><br>
</span><b><i><span style="color:black">If the NXR contributed to your work please cite us in your papers.</span></i></b><span style="font-size:11.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><b><i><span style="color:black">Cite us in Materials and Methods as National Xenopus Resource RRID:SCR_013731 at the Marine Biological Laboratory</span></i></b><span style="font-size:11.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><b><i><span style="color:black">Cite us in your references: </span>
</i></b><b><i>Horb M. et. al. (2019). Xenopus resources: transgenic, inbred and mutant animals, training opportunities, and web-based support. Front Physiol. 10:387. PMCID: PMC6497014</i></b><span style="font-size:11.0pt;font-family:"Arial",sans-serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">---------------------------------------------------------------------------------------<br>
Marko Horb, Ph.D. |Director, National <i>Xenopus</i> Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering<br>
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">Email: </span><a href="mailto:mhorb@mbl.edu"><span style="font-size:10.5pt;font-family:"Times New Roman",serif">mhorb@mbl.edu</span></a><span lang="FR" style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"> </span><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black">|
Office: 508-289-7627 | Lab: 508-289-7370 | Cell: 508-564-3764</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><a href="http://www.mbl.edu/horb"><span style="font-size:10.5pt;font-family:"Times New Roman",serif">http://www.mbl.edu/horb</span></a><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"><br>
</span><a href="http://www.mbl.edu/xenopus"><span style="font-size:10.5pt;font-family:"Times New Roman",serif">http://www.mbl.edu/xenopus</span></a><span style="font-size:10.5pt;font-family:"Times New Roman",serif;color:black"><br>
</span><a href="https://twitter.com/xenopusnxr"><span style="font-size:10.5pt;font-family:"Times New Roman",serif">Twitter</span></a><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</body>
</html>