<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Dear Leon,<div class=""><br class=""></div><div class="">The most time consuming part is removing the vitelline membranes. When I was animal cap king decades ago my preferred method was to use an eye brow hair knife and put the de-vitellinised blastula embryos on their side and decapitate them with the eye brow knife… a very rapid method. If working with another, I would suggest having someone removing the vitelline membranes and another person performing the decapitations, to ensure consistency in animal cap removals. One can easily get through hundreds this way. How many do you need?<div class=""><br class=""></div><div class="">Enrique</div><div class=""><br class=""><div class="">
<div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div>——————————————————————————————</div><div>Professor Enrique Amaya</div><div>Division of Cell Matrix Biology & Regenerative Medicine</div><div>Michael Smith Building</div><div>School of Biological Sciences</div><div>Faculty of Biology, Medicine and Health</div><div>University of Manchester</div><div>Oxford Road</div><div>Manchester M13 9PT</div><div>United Kingdom</div><div>Office: +44 161 275 1716</div><div>Twitter: @Enrique1Amaya</div></div>
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<div><br class=""><blockquote type="cite" class=""><div class="">On 29 Oct 2020, at 11:08, Green, Jeremy <<a href="mailto:jeremy.green@kcl.ac.uk" class="">jeremy.green@kcl.ac.uk</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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Dear Leon,
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<div class="">With a bit of practice and well-sharpened Dumont #5 forceps, a person can cut around 200 caps in an hour. I suggest that if you want to get many hundreds of caps that you simply have a cap-cutting jamboree - not so much biting the bullet as having
a party. Seriously. I think you’ll have no problem getting volunteers and (COVID, etc. permitting), I’d be happy to come over myself.</div>
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<div class="">Best wishes,</div>
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<div class="">Jeremy</div>
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<div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
Jeremy B.A. Green Ph.D.</div>
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Vice-Dean International (Research)</div>
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Professor of Developmental Biology</div>
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Centre for Craniofacial & Regenerative Biology</div>
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King's College London</div>
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Guy's Tower, Floor 27</div>
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London SE1 9RT</div>
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UK</div>
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<br class="">
<a href="mailto:jeremy.green@kcl.ac.uk" class="">jeremy.green@kcl.ac.uk</a><br class="">
Tel. +44 20 7188 1794<br class="">
<a href="https://www.kcl.ac.uk/people/jeremy-green" class="">https://www.kcl.ac.uk/people/jeremy-green</a><br class="">
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<div class="">On 29 Oct 2020, at 03:45, Leon Peshkin <<a href="mailto:peshkin@gmail.com" class="">peshkin@gmail.com</a>> wrote:</div>
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<div class="">Dear Colleagues !</div>
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<div class="">I have a few general questions and tried posting to Slack "Xenopus Community" without success. People - Slack is a nice way to keep discussions going, please use it !
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<div class="">Has someone worked out a way to make animal caps en masse ? I did hear the gastromaster legend and even obtained one with a few tips … but it does not really work in my hands. Any other devices ? Please do not tell me to just bite the bullet.
I'd like to be able to cut many hundreds. <br class="">
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<div class=""> There are a couple of animal cap instructional videos with forceps but I know many people use other techniques. Would be nice to have more videos.
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<div class="">Does someone have a protocol and experience for blood perfusion in Xenopus ?
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<div class=""> THANK YOU <br class="">
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<div class=""> - L. Peshkin <br class="">
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