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Hi all,
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<div class="">It’s nice to know that my name is now invoked in mystical legends…..</div>
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<div class="">As it happens, we TRIED to make the gastromaster work for en mass cutting of caps, but we were not successful. Ultimately, the tips get gummed.</div>
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<div class="">Leon, I know you don’t wanna hear it, but…just bite the bullet. My folks now routinely cut >1000 caps or DMZs in a single session, but they do it the old fashioned way: Three people, six pairs of Inox #5, three stereoscopes, and several hours….. </div>
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<div class="">And sorry, I don’t do Slack.</div>
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<div class="">-jbw</div>
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<font size="2" class=""><span style="font-style: normal;" class="">John Wallingford</span></font></div>
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<font size="2" class=""><span style="font-style: normal;" class="">William and Gwyn Shive Endowed Professor</span></font></div>
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<font size="2" class=""><span style="font-style: normal;" class="">Dept. of Molecular Biosciences</span></font></div>
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<font size="2" class=""><span style="font-style: normal;" class="">University of Texas at Austin</span></font></div>
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<font size="2" class=""><span style="font-style: normal;" class="">512-232-2784</span></font></div>
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<font size="2" class=""><span style="font-style: normal;" class=""><a href="mailto:wallingford@austin.utexas.edu" class="">wallingford@austin.utexas.edu</a></span></font></div>
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<div class="">On Oct 29, 2020, at 2:50 PM, Peter Vize <<a href="mailto:pvize@ucalgary.ca" class="">pvize@ucalgary.ca</a>> wrote:</div>
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Leon</span>
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Yes, they have. John Wallingford and Ed Marcotte scaled it up a few years ago to do protein:protein work. I’m sure John can provide the details. They use gastromasters and worked out how to make their own tips.</div>
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Slack takes over your life and I avoid it like the plague…</div>
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Peter</div>
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Dr. Peter Vize<br class="">
Professor Emeritus<br class="">
Department of Biological Sciences<br class="">
University of Calgary<br class="">
2500 University Drive NW<br class="">
Calgary, Alberta T2N 1N4<br class="">
Canada<br class="">
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ph +61 401 300 965<br class="">
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<a href="http://www.vizelab.com/" class="">http://www.vizelab.com/</a></div>
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<div class="">On Oct 29, 2020, at 2:45 PM, Leon Peshkin <<a href="mailto:peshkin@gmail.com" class="">peshkin@gmail.com</a>> wrote:</div>
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<td class="" style="color: rgb(255, 0, 0);">[△EXTERNAL]</td>
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<div class="">Dear Colleagues !</div>
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<div class="">I have a few general questions and tried posting to Slack "Xenopus Community" without success. People - Slack is a nice way to keep discussions going, please use it !<span class="Apple-converted-space"> </span><br class="">
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<div class="">Has someone worked out a way to make animal caps en masse ? I did hear the gastromaster legend and even obtained one with a few tips … but it does not really work in my hands. Any other devices ? Please do not tell me to just bite the bullet.
I'd like to be able to cut many hundreds.<span class="Apple-converted-space"> </span><br class="">
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<div class=""> There are a couple of animal cap instructional videos with forceps but I know many people use other techniques. Would be nice to have more videos.<span class="Apple-converted-space"> </span><br class="">
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<div class="">Does someone have a protocol and experience for blood perfusion in Xenopus ?<span class="Apple-converted-space"> </span><br class="">
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<div class=""> THANK YOU<span class="Apple-converted-space"> </span><br class="">
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<div class=""> - L. Peshkin<span class="Apple-converted-space"> </span><br class="">
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