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<p class="MsoNormal">Dear Colleagues,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Greetings and salutations to all.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We are asking for your assistance to promote the 2022 Xenopus Course at Cold Spring Harbor Laboratory. The Xenopus course at CSHL has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit
Xenopus for understanding cell and developmental biology. After a pause following the Covid-19 pandemic, we are happy to announce that the course is back for in-person instruction next year. CSHL will work closely with us to take precautionary measures to
ensure a safe learning environment.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest. We will design gRNAs for these genes to allow the students to knock these genes out at the course
and analyze any phenotypes using all the tools available, from cut-and-paste embryology to advanced high-speed fluorescence confocal imaging.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSHL. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success
in the previous years and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><b>Important Dates:<o:p></o:p></b></p>
<p class="MsoNormal"><b>Course – March 30-April 12, 2022<o:p></o:p></b></p>
<p class="MsoNormal"><b>Application Due Date: January 31, 2022<o:p></o:p></b></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have an exciting list of speakers/instructors including:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><a name="OLE_LINK1">Lance Davidson, University of Pittsburgh;<o:p></o:p></a></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Chenbei Chang, University of Alabama at Birmingham;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Hiro Funabiki, The Rockefeller University;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Douglas Houston, University of Iowa;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Mustafa Khokha, Yale University;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Carole LaBonne, Northwestern University;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Karen Liu, King’s College London, UK;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Roberto Mayor, University College London, UK;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Rachel Miller, University of Texas;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Brian Mitchell, Northwestern University;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Gert Jan Veenstra, Radboud University, Netherland;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Sarah Woolner, University of Manchester, UK;<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-bookmark:OLE_LINK1">Martin Wuhr, Princeton University</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">For more information and application submission, please visit the website
<a href="https://meetings.cshl.edu/courses.aspx?course=C-XENO&year=22">https://meetings.cshl.edu/courses.aspx?course=C-XENO&year=22</a>.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks,<o:p></o:p></p>
<p class="MsoNormal">Lance Davidson, University of Pittsburgh<o:p></o:p></p>
<p class="MsoNormal">Chenbei Chang, University of Alabama at Birmingham<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Course objectives:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more. In situ hybridization, immunohistochemistry, CRISPR, and MO loss of function.<o:p></o:p></p>
<p class="MsoNormal">Live cell and embryo Imaging and fluorescence - confocal microscopy, image analysis and figure design. Genomic and proteomic approaches to development, regeneration, and disease models.<o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
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