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<div class="">Dear Colleagues</div>
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<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);">If you have old(-er) frogs in your colonies, these are very valuable to our developing research agenda on "aging biology in frogs". </div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);">Please get in touch with Leon Peshkin (<a href="mailto:peshkin@gmail.com" class="">peshkin@gmail.com</a>, SUBJECT: OLD FROGS) if you are planning to sacrifice an old frog, we would ether
try to get it from you or ask you to take some quick biopsies / tissue snips for us. Of interest are X.laevis frogs older than 10 years and X.tropicalis older than 6 years, but of particular value X.laevis older than 20 or X.trops older than 10. If there
is some uncertainty about the age but you have a solid guess that is also good. We also appreciate any anecdotes about frogs longevity, health and fertility. Here is our first preprint on so-called DNA methylation clocks which are likely the first such clocks
outside of mammals: </div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);">"<span class="">Age-associated DNA methylation changes in Xenopus frogs" </span></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><a href="https://www.biorxiv.org/content/10.1101/2022.07.22.500491v1" class="">https://www.biorxiv.org/content/10.1101/2022.07.22.500491v1</a></div>
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<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);">As Marko mentioned, Peshkin@HMS and Horb@MBL got funded to create "Tabula Rana" - the frog cell type atlas. This is meant to be a highly collaborative effort with Xenbase and all of you.
We are presently setting up single-cell platforms to be used at the NXR with the assistance of a trained technician. We are thinking of bringing 15-20 people together to MBL for a Xenopus Jamboree 2.0 <span class="">(see </span><font color="#000000" class=""><a href="https://kleintools.hms.harvard.edu/tools/tree_data/Xenopus/Jamboree.Genesis.dvg.23383.pdf" class="">https://kleintools.hms.harvard.edu/tools/tree_data/Xenopus/Jamboree.Genesis.dvg.23383.pdf</a>)
to dissect tissues from embryos and</font></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><font color="#000000" class="">adults in a consistent way, assisted by technicians, to profile everything with multiple technologies in parallel and get a massive push for Tabula Rana. This
will also be a boot camp to traian people in single cell and new satellite </font>protocols. Please get in touch with <span class="">Leon Peshkin (<a href="mailto:peshkin@gmail.com" class="">peshkin@gmail.com</a>, </span>SUBJECT: Tabula Rana<span class="">)</span> with
your input on: </div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><span class=""> - Would you like to help organize and fund Xenopus Jamboree 2.0 ?</span></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><font color="#000000" class=""> - Would you like to participate if </font><span class="">Jamboree 2.0 eventually takes place?</span></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><span class=""> - Would you like to build a training aspect into Jamboree 2.0 - which single cell tech you know and which do you want to learn ?</span></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><span class=""> - Would you like to participate in a focused working group (or be a solitary curator) for a specific tissue, which one ? </span></div>
<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><span class=""> - Any other idea of how to organize Tabula Rana to be most beneficial to the whole Xenopus community and beyond ...</span></div>
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<div class="" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"><span class="">Leon Peshkin & Marko Horb </span></div>
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