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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Hi Leon,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Yes I’d be very interested in participating in a Jamboree 2.0, especially since we will be also doing some single cell RNA-seq soon.
I’d be happy to participate or chair a focus group on placodes (or neural plate border tissues). Unfortunately , I can’t help with funding but would be happy to help with aspects of organization of the workshop if needed.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Best wishes<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Gerhard<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif"> xenopus-bounces@lists.mbl.edu <xenopus-bounces@lists.mbl.edu>
<b>On Behalf Of </b>Peshkin, Leon<br>
<b>Sent:</b> Wednesday 27 July 2022 19:09<br>
<b>To:</b> xenopus@lists.mbl.edu<br>
<b>Subject:</b> [Xenopus] we want your old frogs and your input into Tabula Rana<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="background:lightyellow"><b>EXTERNAL EMAIL:</b> This email originated outside NUI Galway. Do not open attachments or click on links unless you believe the content is safe.
<br>
<b>RÍOMHPHOST SEACHTRACH:</b> Níor tháinig an ríomhphost seo ó OÉ Gaillimh. Ná hoscail ceangaltáin agus ná cliceáil ar naisc mura gcreideann tú go bhfuil an t-ábhar sábháilte.
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<p class="MsoNormal">Dear Colleagues<o:p></o:p></p>
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<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="color:black">If you have old(-er) frogs in your colonies, these are very valuable to our developing research agenda on "aging biology in frogs". <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black">Please get in touch with Leon Peshkin (<a href="mailto:peshkin@gmail.com">peshkin@gmail.com</a>, SUBJECT: OLD FROGS) if you are planning to sacrifice an old frog, we would ether try to get it from you or ask you
to take some quick biopsies / tissue snips for us. Of interest are X.laevis frogs older than 10 years and X.tropicalis older than 6 years, but of particular value X.laevis older than 20 or X.trops older than 10. If there is some uncertainty about the age
but you have a solid guess that is also good. We also appreciate any anecdotes about frogs longevity, health and fertility. Here is our first preprint on so-called DNA methylation clocks which are likely the first such clocks outside of mammals: <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black">"Age-associated DNA methylation changes in Xenopus frogs" <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"><a href="https://www.biorxiv.org/content/10.1101/2022.07.22.500491v1">https://www.biorxiv.org/content/10.1101/2022.07.22.500491v1</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="color:black">As Marko mentioned, Peshkin@HMS and Horb@MBL got funded to create "Tabula Rana" - the frog cell type atlas. This is meant to be a highly collaborative effort with Xenbase and all of you. We are presently setting
up single-cell platforms to be used at the NXR with the assistance of a trained technician. We are thinking of bringing 15-20 people together to MBL for a Xenopus Jamboree 2.0 (see <a href="https://kleintools.hms.harvard.edu/tools/tree_data/Xenopus/Jamboree.Genesis.dvg.23383.pdf">https://kleintools.hms.harvard.edu/tools/tree_data/Xenopus/Jamboree.Genesis.dvg.23383.pdf</a>)
to dissect tissues from embryos and<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black">adults in a consistent way, assisted by technicians, to profile everything with multiple technologies in parallel and get a massive push for Tabula Rana. This will also be a boot camp to traian people in single
cell and new satellite protocols. Please get in touch with Leon Peshkin (<a href="mailto:peshkin@gmail.com">peshkin@gmail.com</a>, SUBJECT: Tabula Rana) with your input on: <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> - Would you like to help organize and fund Xenopus Jamboree 2.0 ?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> - Would you like to participate if Jamboree 2.0 eventually takes place?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> - Would you like to build a training aspect into Jamboree 2.0 - which single cell tech you know and which do you want to learn ?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> - Would you like to participate in a focused working group (or be a solitary curator) for a specific tissue, which one ? <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> - Any other idea of how to organize Tabula Rana to be most beneficial to the whole Xenopus community and beyond ...<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="color:black">Leon Peshkin & Marko Horb <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
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