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<p class="MsoNormal">Dear Colleagues,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Happy New Year!<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We hope that you are having a great start of the new year. We are asking for your assistance to promote the 2023 Xenopus Course at Cold Spring Harbor Laboratory. The Xenopus course at CSHL has a long tradition of training the next generation
of Xenopus researchers in critical skills to exploit Xenopus for understanding cell and developmental biology. After a pause following the Covid-19 pandemic, we have re-started the in-person instruction last year and will continue the in-person format in 2023.
CSHL will work closely with us to take precautionary measures to ensure a safe learning environment.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest. We will design gRNAs for these genes to allow the students to knock these genes out at the course
and analyze any phenotypes using all the tools available, from cut-and-paste embryology to advanced high-speed fluorescence confocal imaging.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSHL. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success
in the previous years and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We would like to emphasize that most of the students will receive financial support from CSHL to participate in the course.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><b>Important Dates:<o:p></o:p></b></p>
<p class="MsoNormal"><b>Course – 12 April to 25 April, 2023<o:p></o:p></b></p>
<p class="MsoNormal"><b>Application Due Date: 31 January, 2023<o:p></o:p></b></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have an exciting list of speakers/instructors including:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Lance Davidson, University of Pittsburgh;<o:p></o:p></p>
<p class="MsoNormal">Chenbei Chang, University of Alabama at Birmingham;<o:p></o:p></p>
<p class="MsoNormal">Hiro Funabiki, The Rockefeller University;<o:p></o:p></p>
<p class="MsoNormal">Douglas Houston, University of Iowa;<o:p></o:p></p>
<p class="MsoNormal">Mustafa Khokha, Yale University;<o:p></o:p></p>
<p class="MsoNormal">Rachel Miller, University of Texas;<o:p></o:p></p>
<p class="MsoNormal">Brian Mitchell, Northwestern University;<o:p></o:p></p>
<p class="MsoNormal">Anne-Helene Monsoro-Burq, Institut Curie, France;<o:p></o:p></p>
<p class="MsoNormal">Nanette Nascone-Yoder, North Carolina State University;<o:p></o:p></p>
<p class="MsoNormal">Asako Shindo, Kumomoto University, Japan;<o:p></o:p></p>
<p class="MsoNormal">Hellen Willsey, University of California at San Francisco;<o:p></o:p></p>
<p class="MsoNormal">Sarah Woolner, University of Manchester, UK<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">For more information and application submission, please visit the website
<a href="https://meetings.cshl.edu/courses.aspx?course=C-XENO&year=23">https://meetings.cshl.edu/courses.aspx?course=C-XENO&year=23</a>.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks,<o:p></o:p></p>
<p class="MsoNormal">Lance Davidson, University of Pittsburgh<o:p></o:p></p>
<p class="MsoNormal">Chenbei Chang, University of Alabama at Birmingham<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Course objectives:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more. In situ hybridization, immunohistochemistry, CRISPR, and MO loss of function.<o:p></o:p></p>
<p class="MsoNormal">Live cell and embryo Imaging and fluorescence - confocal microscopy, image analysis and figure design. Genomic and proteomic approaches to development, regeneration, and disease models.<o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
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