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The only issue with used PLI 100 units is there are no spare parts. You can only replace tubing, frequently cracked. Harvard will not take them for service. I have a colleague who bought two cheap and assembled one working unit from those.
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> xenopus-bounces@lists.mbl.edu <xenopus-bounces@lists.mbl.edu> on behalf of Davidson, Lance <lad43@pitt.edu><br>
<b>Sent:</b> Tuesday, March 14, 2023 4:46:42 PM<br>
<b>To:</b> xenopus@lists.mbl.edu <xenopus@lists.mbl.edu><br>
<b>Subject:</b> Re: [Xenopus] microinjector</font>
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<div class="PlainText">We have had good luck with used PLI-100A from Ebay (Warner, Harvard Apparatus, Medical Systems). They remain functional and get frequent use.<br>
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Lance<br>
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From: xenopus-bounces@lists.mbl.edu <xenopus-bounces@lists.mbl.edu> On Behalf Of xenopus-request@lists.mbl.edu<br>
Sent: Tuesday, March 14, 2023 2:48 PM<br>
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Subject: Xenopus Digest, Vol 118, Issue 5<br>
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Today's Topics:<br>
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1. Re: microinjector (Chang, Chenbei)<br>
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Message: 1<br>
Date: Tue, 14 Mar 2023 18:29:51 +0000<br>
From: "Chang, Chenbei" <cchang@uab.edu><br>
Subject: Re: [Xenopus] microinjector<br>
To: National Xenopus Resource <xenopus@mbl.edu>,<br>
"xenopus@lists.mbl.edu" <xenopus@lists.mbl.edu><br>
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We recently got an injector PM1000 from MicroData Instrument (see attached catalog). It can both fill and inject. There is only one foot pedal, so you have to toggle between fill and inject constantly. However, once you get used to it, it works quite well.
It is much cheaper than Warner Instruments device. The other choice is Narishige. It is more expensive than MDI injector too.<br>
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From: xenopus-bounces@lists.mbl.edu <xenopus-bounces@lists.mbl.edu> On Behalf Of National Xenopus Resource<br>
Sent: Tuesday, March 14, 2023 1:03 PM<br>
To: xenopus@lists.mbl.edu<br>
Subject: [Xenopus] FW: microinjector<br>
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Jan tried to send this email to the list and for some reason it bounced so I am forwarding it to you all<br>
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Marko<br>
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From: Jan Christian <jan.christian@neuro.utah.edu<mailto:jan.christian@neuro.utah.edu>><br>
Date: Tuesday, March 14, 2023 at 1:44 PM<br>
To: xenopus-bounces@lists.mbl.edu<mailto:xenopus-bounces@lists.mbl.edu> <xenopus-bounces@lists.mbl.edu<mailto:xenopus-bounces@lists.mbl.edu>>, National Xenopus Resource <xenopus@mbl.edu<mailto:xenopus@mbl.edu>><br>
Subject: microinjector<br>
Hi,<br>
We are needing to replace one of our Warner instruments PLI-100A microinjectors and apparently these are no longer made (replaced by the AI Roboinjector that can inject up to 4 samples per well in 386 well dishes!!!). Anyone have any suggestions for a replacement
that has both fill and inject capabilities. We do a lot of blastocoele aspirations so need the fill feature.<br>
<br>
Thanks,<br>
Jan<br>
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