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<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Georgia",serif;color:black"><img width="582" height="125" style="width:6.0625in;height:1.302in" id="Picture_x0020_1" src="cid:image001.png@01DAC7A9.0ABCD980" alt="A black background with a letter xDescription automatically generated"></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:20.0pt;font-family:"Georgia",serif;color:#3A7C22">Newsletter Summer 2024</span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:14.0pt"> </span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:red">NEW </span><span style="font-size:14.0pt;color:#3A7C22">JBrowse2 on Xenbase</span></p>
<p class="MsoNormal" style="text-align:justify"><b>JBrowse2</b> is now in <span style="color:black">
beta testing </span>on Xenbase, with <span class="grame">the v10.1</span> <i>X. laevis</i> genome,
<span class="spelle">CRISPRscan</span> gRNA tracks and Xenbase catalogs of published gRNAs and MOs already loaded. Coming next …
<span class="grame">the <i>X.</i></span><i> tropicali</i>s v10.0 genome. <b>Find JBrowse2 on Xenbase via the Genomes menu on the homepage.</b></p>
<p class="MsoNormal" style="text-align:justify">One of the big improvements of JBrowse2 is the ability to view 2 genomes
<span style="color:black">simultaneously</span>. This feature allows you to view linear synteny between
<i>X. tropicalis, X. <span class="spelle">laevis.L</span> </i>and<i> X. <span class="spelle">
laevis.S</span></i> for each chromosome. A Quick Tutorial video demonstrating this powerful feature will be available soon<span style="color:#1C1E21">!</span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22">2024 <i>Xenopus</i> researcher Survey</span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22">Open now! Tell us what you really think!</span></p>
<p class="MsoNormal">We want to hear from you about how you use Xenbase. Everyone’s opinion is valuable- all
<i>Xenopus</i> peeps <span class="grame">from teaching</span> and research labs, undergraduates, trainees, faculty, adjuncts and staff scientists. Everyone is invited to opine.</p>
<p class="MsoNormal">Please take our new ‘24 survey today- just click here: <a href="https://forms.gle/5WFANQTKA6TuSyg67">
https://forms.gle/5WFANQTKA6TuSyg67</a></p>
<p class="MsoNormal"><span style="font-size:14.0pt"> </span>
Or scan this QR code<span style="font-size:14.0pt">. </span></p>
<p class="MsoNormal" style="margin-left:1.0in"><span style="font-size:14.0pt"><img border="0" width="156" height="153" style="width:1.625in;height:1.5937in" id="Picture_x0020_2" src="cid:image002.png@01DAC7A9.13BFD370" alt="A qr code with a frog on it
Description automatically generated"></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt"> </span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22">New mutant Lines @ the NXR + CRISPR support grows on
<span class="grame">Xenbase</span></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="color:black">The NXR now has 126 mutant
<i>X. tropicalis</i> and <i>X. laevis</i> lines available to order, with several <span class="spelle">
mutatnts</span> available in both species. More CRISPR knockout lines are being developed. The Xenbase gRNA catalog now has 515 gRNAs used by the NXR and in published research, plus
<span class="spelle">JBrowse</span> and JBrowse2 both have a <span class="spelle">
CRISPRscan</span> track of all predicted Cas9 gRNAs against the whole genome and against coding regions.</span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22"> </span></p>
<p class="MsoNormal"><span style="color:#3B7D23">Xenbase has new computer servers with upgraded VMware operating system, so it is now even faster and more reliable than ever. We hope you notice the faster BLAST operations,
<span class="grame">searches</span> and page load times.</span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22"> </span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;color:#3A7C22">Summer and Fall Meetings and Conferences:
</span></p>
<p class="MsoNormal" style="text-align:justify"><b>4EAC</b>, the <a href="https://amphiclub2024.sciencesconf.org/">
<b>4<sup>th</sup> European Amphibian Club</b></a> meetings gets under way in Bordeaux France July 1st-3<sup>rd</sup>. Xenbase PIs will be there, and we look forward to seeing & hearing great science from our many colleagues and friends in the
<i>Xenopus</i> community. <b>SDB:</b> The <a href="https://www.sdbonline.org/2024mtg">
<b>83<sup>rd</sup> annual SDB Meeting</b></a> (Atlanta, Georgia, July 14-17th) and
<a href="https://midwestsdb.weebly.com/"><b>Midwest SDB</b></a> meetings (Madison, Wisconsin, August 11-13<sup>th</sup><b>)
</b> are a great opportunity to promote the excellent <i>Xenopus</i> research being conducted to a broader audience. Hope to see you there!
<b>XRET,</b> the <i>Xenopus</i> Resources and Emerging Technologies meeting will be held Oct 11th-14th at the MBL, hosted by Marko
<span class="spelle">Horb</span> [NXR Director and chief frog wrangler] and his staff. Watch Xenbase homepage for link to registration!</p>
<p class="MsoNormal"> </p>
<p class="MsoNormal">Need Help? Have Questions? Contact us via the Xenbase help desk:
<span style="color:#3B7D23"><a href="mailto:Xenbase@cchmc.org"><span style="color:#355A64">Xenbase@cchmc.org</span></a></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Georgia",serif;color:black"> </span></p>
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