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<p>Dear colleagues,</p>
<p>as I mentioned in the last XRET meeting (for those who attended)
we are planning to introduce an <b>integration/exchange cassette
in a safe harbor locus</b> in Xenopus. The idea is that a line
containing this cassette can be used for locus-controlled
single-copy integration of transgenes.</p>
<p>In the vector we intend to introduce <b>LoxP </b>and <b>FRT </b>sites
for Cre and Flp recombination mediated cassette exchange (<b>RMCE</b>)
but will also include a <b>I-Sce1, PhiC31</b> and <b>Bxb1</b>
integration site. <br>
</p>
<p>1) Are you thinking about other sites/sequences that should be
included?</p>
<p>2) Concerning use of fluorescent reference gene, I was wondering
whether next to GFP, there are other fluorescent proteins that
have good antibodies that are compatible with methanol fixation?</p>
<p>Thanks for your answers at <b><a class="moz-txt-link-abbreviated" href="mailto:Kris.Vleminckx@irc.ugent.be">Kris.Vleminckx@irc.ugent.be</a></b></p>
<p>Kris<br>
</p>
<pre class="moz-signature" cols="72">--
Kris Vleminckx, PhD
Ghent University
Department of Biomedical Molecular Biology
Technologiepark 71
9052 Ghent
Belgium
Tel: +32 9 33 13 760
Email: <a class="moz-txt-link-abbreviated" href="mailto:Kris.Vleminckx@irc.ugent.be">Kris.Vleminckx@irc.ugent.be</a>
<a class="moz-txt-link-freetext" href="https://www.irc.ugent.be/index.php?id=krisvleminckxhome">https://www.irc.ugent.be/index.php?id=krisvleminckxhome</a></pre>
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