From gerhard.schlosser at nuigalway.ie  Mon Aug  8 06:58:01 2022
From: gerhard.schlosser at nuigalway.ie (Schlosser, Gerhard)
Date: Mon, 8 Aug 2022 10:58:01 +0000
Subject: [Xenopus] we want your old frogs and your input into Tabula Rana
In-Reply-To: <2B3CFB7C-8BE4-4C66-849A-6FBD0A88CA69@hms.harvard.edu>
References: <2B3CFB7C-8BE4-4C66-849A-6FBD0A88CA69@hms.harvard.edu>
Message-ID: <DB8PR01MB6154F424B88B231107C2D1A082639@DB8PR01MB6154.eurprd01.prod.exchangelabs.com>

Hi Leon,

Yes I?d be very interested in participating in a Jamboree 2.0, especially since we will be also doing some single cell RNA-seq soon. I?d be happy to participate or chair a focus group on placodes (or neural plate border tissues). Unfortunately , I can?t help with funding but would be happy to help with aspects of organization of the workshop if needed.

Best wishes

Gerhard

From: xenopus-bounces at lists.mbl.edu <xenopus-bounces at lists.mbl.edu> On Behalf Of Peshkin, Leon
Sent: Wednesday 27 July 2022 19:09
To: xenopus at lists.mbl.edu
Subject: [Xenopus] we want your old frogs and your input into Tabula Rana

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Dear Colleagues

If you have old(-er) frogs in your colonies, these are very valuable to our developing research agenda on "aging biology in frogs".
Please get in touch with Leon Peshkin (peshkin at gmail.com<mailto:peshkin at gmail.com>, SUBJECT: OLD FROGS) if you are planning to sacrifice an old frog, we would ether try to get it from you or ask you to take some quick biopsies / tissue snips for us.  Of interest are X.laevis frogs older than 10 years and X.tropicalis older than 6 years, but of particular value X.laevis older than 20 or X.trops older than 10. If there is some uncertainty about the age but you have a solid guess that is also good. We also appreciate any anecdotes about frogs longevity, health and fertility. Here is our first preprint on so-called DNA methylation clocks which are likely the first such clocks outside of mammals:
"Age-associated DNA methylation changes in Xenopus frogs"
https://www.biorxiv.org/content/10.1101/2022.07.22.500491v1

As Marko mentioned, Peshkin at HMS and Horb at MBL got funded to create "Tabula Rana" - the frog cell type atlas. This is meant to be a highly collaborative effort with Xenbase and all of you. We are presently setting up single-cell platforms to be used at the NXR with the assistance of a trained technician. We are thinking of bringing 15-20 people together to MBL for a Xenopus Jamboree 2.0 (see https://kleintools.hms.harvard.edu/tools/tree_data/Xenopus/Jamboree.Genesis.dvg.23383.pdf)  to dissect tissues from embryos and
adults in a consistent way, assisted by technicians, to profile everything with multiple technologies in parallel and get a massive push for Tabula Rana. This will also be a boot camp to traian people in single cell and new satellite protocols.  Please get in touch with Leon Peshkin (peshkin at gmail.com<mailto:peshkin at gmail.com>, SUBJECT: Tabula Rana) with your input on:
 - Would you like to help organize and fund Xenopus Jamboree 2.0 ?
 - Would you like to participate if Jamboree 2.0 eventually takes place?
 - Would you like to build a training aspect into Jamboree 2.0 - which single cell tech you know and which do you want to learn ?
 - Would you like to participate in a focused working group (or be a solitary curator) for a specific tissue, which one ?
 - Any other idea of how to organize Tabula Rana to be most beneficial to the whole Xenopus community and beyond ...

Leon Peshkin & Marko Horb

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From xenopus at mbl.edu  Tue Aug 16 13:38:49 2022
From: xenopus at mbl.edu (National Xenopus Resource)
Date: Tue, 16 Aug 2022 17:38:49 +0000
Subject: [Xenopus] Other Xenopus species
Message-ID: <BLAPR22MB2275448E0B010B2389D30A87A96B9@BLAPR22MB2275.namprd22.prod.outlook.com>

Dear Xenopus community
            A quick email to inform you that we now have additional Xenopus species. We do not have many so let us know in advance if you want some so we can breed more.
Xenopus borealis
Xenopus petersii
Xenopus powerii
Xenopus boumbaensis

marko


---------------------------------------------------------------------------------------
Marko Horb, Ph.D. |Director, National Xenopus Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543
Email: mhorb at mbl.edu<mailto:mhorb at mbl.edu> | Office: 508-289-7627 | Lab: 508-289-7370 | Cell:  508-564-3764

https://www.mbl.edu/research/resources-research-facilities/national-xenopus-resource
Twitter<https://twitter.com/xenopusnxr>

**************************************************************************
If the NXR contributed to your work please acknowledge us in your publications and talks.
Cite us in Materials and Methods as National Xenopus Resource RRID:SCR_013731 at the Marine Biological Laboratory and in the Acknowledgements you can just mention the National Xenopus Resource.
Cite us in your references: Horb M. et. al. (2019). Xenopus resources: transgenic, inbred and mutant animals, training opportunities, and web-based support. Front Physiol. 10:387. PMCID: PMC6497014


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