[Xenopus] alternatives for obtaining freshly fertilized eggs?

[Bob Chow]

Hi,

My animal care committee is wanting me to look for alternatives to housing female Xenopus used to generate embryos for my undergraduate developmental biology course lab (we are switching to MBL cryo’d sperm). We run two labs a year: one where we investigate fertilization and perform polyspermic fertilizations. The other we perform cell dissociation and Spemann organizer transplant.

Are there any companies that deliver ready to fertilize eggs? If so, how reliable is this type of approach in terms of yield and quality?

This is the closest thing that I have found:

https://www.wardsci.com/store/product/14683253/ward-s-prepared-xenopus-laevis-reproductive-tissue

Any feedback would be appreciated.

Thank you!
Bob
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