[Xenopus] 2024 CSHL Xenopus course

[Chang, Chenbei]

Dear Colleagues,

Happy New Year!

We hope that you are having a great start of the new year.  We are asking for your assistance to promote the 2024 Xenopus Course at Cold Spring Harbor Laboratory. The Xenopus course at CSHL has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for understanding cell and developmental biology. After a pause following the Covid-19 pandemic, we have resumed the in-person instruction in 2022 and 2023. We will continue our in-person course this year. CSHL will work closely with us to take precautionary measures to ensure a safe learning environment.

To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest. We will design gRNAs for these genes to allow the students to knock these genes out at the course and analyze any phenotypes using all the tools available, from cut-and-paste embryology to advanced high-resolution fluorescence confocal imaging.

In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSHL. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success in the previous years and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.

We would like to emphasize that most of the students will receive financial support from CSHL to participate in the course.

Important Dates:
Course - 2 April to 16 April, 2024
Application Due Date: 31 January, 2024

We have 4 co-instructors and an exciting list of speakers this year, including:

Lance Davidson, University of Pittsburgh;
Chenbei Chang, University of Alabama at Birmingham;
Rachel Miller, University of Texas McGovern Medical School;
Peter Walentek, University of Freiburg, Germany;
Douglas Houston, University of Iowa;
Mustafa Khokha, Yale University;
Hellen Willsey, University of California at San Francisco;
Kris Vleminckx, Gent University, Belgium;
Jennifer Landino, Dartmouth University Geisel School of Medicine;
Jakub Sedzinski, University of Copenhagen, Denmark;
John Young, Simmons University;
Shuyi Nie, Georgia Institute of Technology;
Engin Deniz, Yale University;
Nataliya Zahn, Scientific Illustrator/Artist

We will also introduce various resources available in the Xenopus community.  We thank all the people involved in bringing the information to the course:

Christina James-Zorn, Xenbase, Cincinnati Children's Hospital Medical Center;
Marko Horb, National Xenopus Resource Center, MBL;
Matt Guille, European Xenopus Resource Center, University of Portsmouth;
Hajime Ogino, Amphibian Research Center at Hiroshima University;
Douglas Houston, Antibody resources at DSHB, University of Iowa;
Dominque Alfandari, Antibody development, University of Massachusetts Amherst

For more information and application submission, please visit the website
https://meetings.cshl.edu/courses.aspx?course=C-xeno&year=24
.


Thanks,
Lance Davidson, University of Pittsburgh
Chenbei Chang, University of Alabama at Birmingham
Rachel Miller, University of Texas McGovern Medical School;
Peter Walentek, University of Freiburg, Germany;


Course technical objectives:

Microinjection, microdissection, grafts (Organizer, animal cap, neural crest and more); in situ hybridization, hybridization chain reaction (HCR), immunohistochemistry, CRISPR, MO loss of function; live cell, tissue, and embryo Imaging, fluorescence confocal microscopy, image analysis and figure design; genomic, systems biology and proteomic approaches to development and disease models, including single-cell technology; Xenopus resources.


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