From egb2154 at columbia.edu  Wed Oct  2 15:28:08 2024
From: egb2154 at columbia.edu (Elizabeth Genevieve Bagnato-Conlin)
Date: Wed, 2 Oct 2024 15:28:08 -0400
Subject: [Xenopus] Xine Post -- Kelley Lab
Message-ID: <CAB52qqB0Zs9d_wAfCDw+MU1q987d4oqBp34oPv2R2ZCRxvV+jA@mail.gmail.com>

Hello!

My name is Elizabeth "Bliss" Bagnato-Conlin from the Darcy Kelley Lab at
Columbia, and I'm writing to the community for help with *LHRH dosage*. We
hope to make the switch from hCG to LHRH to induce sexual receptivity in
males and females.

If you have any leads on dosage information, please reach out via email
egb2154 at columbia.edu. Thank you!

-- 
Bliss Bagnato-Conlin (they/them)
Research Assistant | Darcy Kelley Lab
B.A. Neuroscience and Behavior | Columbia University, 2022
email egb2154 at columbia.edu <mh3903 at columbia.edu>
mobile 202-701-5124
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From jeniaya at savion.huji.ac.il  Mon Oct 14 05:10:01 2024
From: jeniaya at savion.huji.ac.il (Jenia Yagil)
Date: Mon, 14 Oct 2024 09:10:01 +0000
Subject: [Xenopus] using pain killers in xenopus
Message-ID: <AS2PR10MB725033336A65F4CA1AB39148B0442@AS2PR10MB7250.EURPRD10.PROD.OUTLOOK.COM>

Hello all
I would like your opinion and experience on the use of pain killers in xenopus.
Is this standard practice in your facility?
What are the drugs of choice, doses and frequency?

Thank you
Jenia


[cid:image001.jpg at 01D7C64C.3C11E9A0]
Dr. Jenia Yagil DVM | veterinarian

Authority for Biological and Biomedical Models
The Hebrew University of Jerusalem
C +972.54.8820724
jeniaya at savion.huji.ac.il<mailto:jeniaya at savion.huji.ac.il>

[cid:image002.png at 01DA46DE.791711D0]



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From Jan.Christian at hsc.utah.edu  Mon Oct 14 12:20:49 2024
From: Jan.Christian at hsc.utah.edu (Jan Christian)
Date: Mon, 14 Oct 2024 16:20:49 +0000
Subject: [Xenopus] using pain killers in xenopus
In-Reply-To: <AS2PR10MB725033336A65F4CA1AB39148B0442@AS2PR10MB7250.EURPRD10.PROD.OUTLOOK.COM>
References: <AS2PR10MB725033336A65F4CA1AB39148B0442@AS2PR10MB7250.EURPRD10.PROD.OUTLOOK.COM>
Message-ID: <DM6PR11MB4754FD4C47A7938BEEB978B5C4442@DM6PR11MB4754.namprd11.prod.outlook.com>

Our vets have disagreed on the need for analgesics but with my last renewal this protocol was agreed to for ovariectomy.  We do not use painkillers for any other procedures.

Drug Generic Name
Flunixin meglumine
Drug Type
Analgesic
Drug Dosage
25 mg/kg
Duration of administration
three days
Frequency of administration*REQUIRED*
Once daily
Route of administration
OTHER
Reason for administration/justification for use
Administered intralymphatically by injection into dorsal lymph sac for pain relief prior to and for 1-2 days post-ovariectomy

Jan


From: xenopus-bounces at lists.mbl.edu <xenopus-bounces at lists.mbl.edu> on behalf of Jenia Yagil <jeniaya at savion.huji.ac.il>
Date: Monday, October 14, 2024 at 9:07?AM
To: xenopus at lists.mbl.edu <xenopus at lists.mbl.edu>
Subject: [Xenopus] using pain killers in xenopus
Hello all
I would like your opinion and experience on the use of pain killers in xenopus.
Is this standard practice in your facility?
What are the drugs of choice, doses and frequency?

Thank you
Jenia


[cid:image001.jpg at 01DB1E31.BBD6AA00]
Dr. Jenia Yagil DVM | veterinarian

Authority for Biological and Biomedical Models
The Hebrew University of Jerusalem
C +972.54.8820724
jeniaya at savion.huji.ac.il<mailto:jeniaya at savion.huji.ac.il>

[cid:image002.png at 01DB1E31.BBD6AA00]



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From clabonne at northwestern.edu  Mon Oct 14 22:07:55 2024
From: clabonne at northwestern.edu (Carole LaBonne)
Date: Tue, 15 Oct 2024 02:07:55 +0000
Subject: [Xenopus] Wow XRET24 was amazing!!!
Message-ID: <87FF8186-79A3-41AC-9E6A-31A52159D150@northwestern.edu>

HUGE kudos to Helen, Jennifer and Coral for organizing such a great meeting. And also to Marko for the amazing job he and his wonderful staff do in supporting our entire community so incredibly and consistently well!!  (and who knew Nikko had such amazing lobster dissection skills???!!!). The future of #Xenopus research is strong and exciting!

______________
Carole LaBonne
Erastus Otis Haven Professor of Life Sciences
Professor, Dept. of Molecular Biosciences
Northwestern University
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From Helen.Willsey at ucsf.edu  Wed Oct 16 00:53:27 2024
From: Helen.Willsey at ucsf.edu (Willsey, Helen)
Date: Wed, 16 Oct 2024 04:53:27 +0000
Subject: [Xenopus] Xenopus twitter: call for content
Message-ID: <SA1PR05MB8660B9658E1153FFF97B017F96462@SA1PR05MB8660.namprd05.prod.outlook.com>

Dear fellow Xenophiles,

I'm taking the helm of the Xenopus twitter account @frogwranglers - huge thanks to John Wallingford for all his work publicising and celebrating all things Xenopus on the account these last years.

So this is a call for CONTENT. Please email me amazing images, movies, gifs, puns, and papers (especially biorxiv since editors look at engagement) celebrating THE FROG!

A big goal is to forever put to rest the myth that one "cannot image in frog" etc etc. As well as to increase education on the amazing advantages of working in frog and our excellent community that celebrates early career scientists. I'll also try and highlight our amazing resources Xenbase and NXR and try to add content that is helpful for even the most experienced xenophile. So, if you don't already, please give the account a follow, tweet at it, and retweet generously.

Cheers

Helen
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From kris.vleminckx at irc.vib-ugent.be  Mon Oct 28 10:31:49 2024
From: kris.vleminckx at irc.vib-ugent.be (Kris Vleminckx)
Date: Mon, 28 Oct 2024 15:31:49 +0100
Subject: [Xenopus] Integrating transgenes in safe harbor locus
Message-ID: <6de92fed-491c-4526-9df8-3085559eada7@irc.ugent.be>

Dear colleagues,

as I mentioned in the last XRET meeting (for those who attended) we are 
planning to introduce an *integration/exchange cassette in a safe harbor 
locus* in Xenopus. The idea is that a line containing this cassette can 
be used for locus-controlled single-copy integration of transgenes.

In the vector we intend to introduce *LoxP *and *FRT *sites for Cre and 
Flp recombination mediated cassette exchange (*RMCE*) but will also 
include a *I-Sce1, PhiC31* and *Bxb1* integration site.

1) Are you thinking about other sites/sequences that should be included?

2) Concerning use of fluorescent reference gene, I was wondering whether 
next to GFP, there are other fluorescent proteins that have good 
antibodies that are compatible with methanol fixation?

Thanks for your answers at *Kris.Vleminckx at irc.ugent.be*

Kris

-- 
Kris Vleminckx, PhD
Ghent University
Department of Biomedical Molecular Biology
Technologiepark 71
9052 Ghent
Belgium
Tel: +32 9 33 13 760
Email:Kris.Vleminckx at irc.ugent.be
https://www.irc.ugent.be/index.php?id=krisvleminckxhome
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