[Physiofaculty] CREST spinning disk

[Brzostowski, Joseph (NIH/NIAID) [E]]

Hi All,

The software stability issues with the CREST system have been addressed.  It was mostly Windows software related.

The system can do three colors 405, 470, 555 with one camera or 470 and 555 with a dual camera set up.

There is stage top incubation and it uses a 60x water lens.

Please be aware that the 555 is a prototype laser and won't work with streaming acquisitions, but timelapses with regular intervals will work fine.  There is a "trick" to try that I can show you if you really must stream with 555.

However stream acquisitions work well with 405 and 470.

Please work with Steven or me for training.

Joe

--
Joseph Brzostowski, Ph.D.
LIG Imaging Facility, Chief
National Institutes of Health/NIAID
12441 Parklawn Dr. Twin II/Rm. 200 E
Rockville, MD 20852

Mobile: 240-599-6364
Cu Wire: 301-761-5024
Fax: 301-402-0259

jb363a at nih.gov
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