[Physiofaculty] Microscope Trainings/Info for Session 3

[Steven Wilbert]

Based on responses so far, I have coordinated the following trainings for
this Saturday (tomorrow).

Please let me know which you and your people are interested in attending so
that I can convey to the trainee and make sure there are participants.

CREST Spinning Disk (dual camera system (green/red), runs metamorph)
Training - Steven

Sat 1-2pm

Nikon Spinning Disk Training/Elements Software Intro - John from Nikon

Sat 2-3pm

Zeiss Widefield Training/Zen Software Intro - Jason/Saige from Zeiss

Sat 3-4pm

Zeiss Spinning Disk - Steven

Sat 5-6pm

As for the OMX and AiryScan, trainings from the manufacturers will need to
be early next week, still coordinating with them. But in the meantime, if
you plan to use these right away next week, I can help in getting started.

Thanks!

On Fri, Jul 15, 2016 at 9:04 AM, Steven Wilbert <swilbert88 at gmail.com>
wrote:

> Hello Session 3 faculty!
>
> Just wanted to send out a reminder email with the available microscope
> systems.
>
> If you plan to use a system heavily for your rotation and would need
> training please set up a time with me and I will coordinate it.
>
> All the systems on our floor are paper signups on the door of the system
> and sign up time is 10pm for the next day.
>
> Shared systems in the basement and Lillie, you will need to sign up with
> PPMS. *You can sign up now for next week and I would suggest doing so as
> these are shared and heavily used by other courses. *
>
> PPMS Login:
> go to: https://ppms.us/mbl/login/?pf=2
> User: Physiology Student
> Pass: mblpass
>
> *Systems Available:*
>
> *Widefield*
>
> Zeiss 1 (full course) – fully automated with incubation and autofocus
>
>
>
> Zeiss 2 (full course) – fully automated with incubation and autofocus
>
>
>
> Zeiss 3 (full course) – fully automated with incubation, and*PhiOptics **Quantitative
> Phase*
>
>
>
> Nikon (full course) – fully automated with incubation, autofocus and *PRIMO
> for protein printing*
>
>
>
> GE DeltaVision (Sessions 1 – 3) – fully automated with incubation (no CO2)
>
>
>
> GE OMX (Sessions 1 – 3) – fully automated with incubation
>
>
>
>
>
> *Spinning Disk Confocal*
>
> Nikon/Yokogawa CSUX1 (full course) – fully automated with incubation.
> With 405 FRAP
>
>
>
> CREST (Sessions 1 – 3) – partially automated with incubation spinning disk
> system from BioVision/89 North on an Olympus base run with MetaMorph
> software.  Note, this system only has a very nice 60x water lens.  Please
> see me or Steven for training.
>
>
>
>
>
> *TIRF*
>
> Nikon (full course) – fully automated with incubation and autofocus
>
>
>
> GE DeltaVision (Sessions 1 – 3) – fully automated with incubation
>
>
>
> GE OMX (Sessions 1 – 3) – fully automated with incubation, ring-TIRF
> version
>
>
> *Super-resolution*
>
> GE OMX (Sessions 1 – 3) Structured Illumination (SIM)– fully automated
> with incubation
>
>
>
>
> *Multi-well plate imaging*
>
> Incucyte (full course) – phase, green and red fluorescence.  System is
> housed in a standard 37°C incubator with CO2.
>
>
> *Microscope systems in basement of Loeb open for the MBL community.  All
> are available for the full course.*
>
>
>
> *Zeiss*
>
> Spinning disk confocal (Yokogawa CSUX1) – fully automated with incubation
> with FRAP and Photoablation from Rapp Opoto.
>
>
>
> 880 with Airyscan and FAST – laser scanning confocal with super-res and
> fast scanning for live cell imaging. Fully automated with incubation
>
>
>
> 800 – laser scanning confocal fully automated with incubation
>
>
>
> Widefield system with and injection apparatus.
>
>
>
> *Nikon*
>
> STORM 4 – super-resolution TIRF system.  Fully automated and will have
> incubation for standard TIRF experiments.  For Session 2 the system will be
> equipped with the CU 3D to improve 3D STORM acquisitions.
>
>
>
> A1R+ - resonance scanning confocal system.  Fully automated with
> incubation and autofocus.
>
> --
> *Steven Wilbert*
> Research Assistant
> Microbiology
> Forsyth Institute
> 585-802-4733
>
>
>
>
>


-- 
*Steven Wilbert*
Research Assistant
Microbiology
Forsyth Institute
585-802-4733
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