[Xenopus] pCS-GAP43-GFP sequence

Sally Moody samoody at gwu.edu
Wed Jun 11 14:02:47 EDT 2014

Hi Jill,
We don't have that sequence, but in our experience it is better to send it
out for sequencing rather than rely on somebody's map because things are
not always what they seem. Since it is in pCS, you can use M13 primers or
better yet SP6 and T7 primers for that plasmid.
Good luck, Sally

On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <johnstoj at ucalgary.ca> wrote:

> Hello,
> We have a construct called pCS-GAP43-GFP here and have no information
> about it.  Does anyone have the sequence that could be sent to us?
> Thanks
> Jillian Johnston
> Research Associate
> Dr. Sarah McFarlane lab
> University of Calgary
> Faculty of Medicine
> Dept of Cell Biology and Anatomy
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> Xenopus at lists.mbl.edu
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Sally A. Moody, Ph.D.
Professor of Anatomy and Regenerative Biology
George Washington University
School of Medicine and Health Sciences
2300 I Street, NW, Washington, DC, 20037, USA
Editor-in-Chief:  *genesis, The Journal of Genetics and Development*
Editor: *Principles of Developmental Genetics, 2nd edition*
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