[Xenopus] pCS-GAP43-GFP sequence
Conlon, Frank L
frank_conlon at med.unc.edu
Wed Jun 11 14:12:35 EDT 2014
Fully agree with Sally.
On Jun 11, 2014, at 2:08 PM, "Sally Moody" <samoody at gwu.edu<mailto:samoody at gwu.edu>> wrote:
Hi Jill,
We don't have that sequence, but in our experience it is better to send it out for sequencing rather than rely on somebody's map because things are not always what they seem. Since it is in pCS, you can use M13 primers or better yet SP6 and T7 primers for that plasmid.
Good luck, Sally
On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <johnstoj at ucalgary.ca<mailto:johnstoj at ucalgary.ca>> wrote:
Hello,
We have a construct called pCS-GAP43-GFP here and have no information
about it. Does anyone have the sequence that could be sent to us?
Thanks
Jillian Johnston
Research Associate
Dr. Sarah McFarlane lab
University of Calgary
Faculty of Medicine
Dept of Cell Biology and Anatomy
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Sally A. Moody, Ph.D.
Professor of Anatomy and Regenerative Biology
George Washington University
School of Medicine and Health Sciences
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202-994-2878
Editor-in-Chief: genesis, The Journal of Genetics and Development
Editor: Principles of Developmental Genetics, 2nd edition
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