[Xenopus] pCS-GAP43-GFP sequence

[Daniel C Weinstein]

Hi Peter: could you post the pCS2-specific "T7" sequence, please?

Thanks,
Dan
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Daniel C. Weinstein
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On Jun 11, 2014, at 2:56 PM, "Peter Klein" <pklein at mail.med.upenn.edu<mailto:pklein at mail.med.upenn.edu>> wrote:

Hi Jill,

I agree with Sally. But the “T7" sequence in pCS2 is not the standard T7 primer sequence, so sequencing facilities may not be able to sequence from that end with their stock primers. I think you need to provide the sequencing facility with a pCS2 appropriate “T7" primer or use T3 (a little farther from the 3’ end). SP6 and T3 are fine.

I think this plasmid might be from Eddy DeRobertis lab, so you can also check with them about it.

Peter


On Jun 11, 2014, at 2:02 PM, Sally Moody <samoody at gwu.edu<mailto:samoody at gwu.edu>> wrote:

Hi Jill,
We don't have that sequence, but in our experience it is better to send it out for sequencing rather than rely on somebody's map because things are not always what they seem. Since it is in pCS, you can use M13 primers or better yet SP6 and T7 primers for that plasmid.
Good luck, Sally


On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <johnstoj at ucalgary.ca<mailto:johnstoj at ucalgary.ca>> wrote:
Hello,

We have a construct called pCS-GAP43-GFP here and have no information
about it.  Does anyone have the sequence that could be sent to us?

Thanks


Jillian Johnston
Research Associate
Dr. Sarah McFarlane lab
University of Calgary
Faculty of Medicine
Dept of Cell Biology and Anatomy




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Sally A. Moody, Ph.D.
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