[Xenopus] pCS-GAP43-GFP sequence

[Peter Klein]

Hi Jill, 

I agree with Sally. But the “T7" sequence in pCS2 is not the standard T7 primer sequence, so sequencing facilities may not be able to sequence from that end with their stock primers. I think you need to provide the sequencing facility with a pCS2 appropriate “T7" primer or use T3 (a little farther from the 3’ end). SP6 and T3 are fine. 

I think this plasmid might be from Eddy DeRobertis lab, so you can also check with them about it.

Peter


On Jun 11, 2014, at 2:02 PM, Sally Moody <samoody at gwu.edu> wrote:

> Hi Jill,
> We don't have that sequence, but in our experience it is better to send it out for sequencing rather than rely on somebody's map because things are not always what they seem. Since it is in pCS, you can use M13 primers or better yet SP6 and T7 primers for that plasmid.
> Good luck, Sally
> 
> 
> On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <johnstoj at ucalgary.ca> wrote:
> Hello,
> 
> We have a construct called pCS-GAP43-GFP here and have no information
> about it.  Does anyone have the sequence that could be sent to us?
> 
> Thanks
> 
> 
> Jillian Johnston
> Research Associate
> Dr. Sarah McFarlane lab
> University of Calgary
> Faculty of Medicine
> Dept of Cell Biology and Anatomy
> 
> 
> 
> 
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu
> https://lists.mbl.edu/mailman/listinfo/xenopus
> 
> 
> 
> -- 
> Sally A. Moody, Ph.D.
> Professor of Anatomy and Regenerative Biology
> George Washington University
> School of Medicine and Health Sciences
> 2300 I Street, NW, Washington, DC, 20037, USA
> 202-994-2878
> Editor-in-Chief:  genesis, The Journal of Genetics and Development
> Editor: Principles of Developmental Genetics, 2nd edition
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu
> https://lists.mbl.edu/mailman/listinfo/xenopus