[Xenopus] pCS-GAP43-GFP sequence

[Daniel C Weinstein]

Thx!

--
Daniel C. Weinstein
Associate Professor
Biology Department
Queens College, CUNY
65-30 Kissena Boulevard<x-apple-data-detectors://0/0>
Queens, NY 11367-1597
718-997-4552<tel:718-997-4552> (office) /4258 (lab)
Fax: 718-559-6745<tel:718-559-6745>
daniel.weinstein at qc.cuny.edu<mailto:daniel.weinstein at qc.cuny.edu>

On Jun 11, 2014, at 3:18 PM, "Takuya Nakayama" <tn8t at virginia.edu<mailto:tn8t at virginia.edu>> wrote:

That's what I was about to point out too.
In case you wonder, here is the "T7" primer which we've been using for
pCS2+ sequencing: 5'-AATACGACTCACTATAG-3' (just shorter version of
original T7).

Takuya


On Jun 11, 2014, at 2:49 PM, Peter Klein wrote:

Hi Jill,

I agree with Sally. But the “T7" sequence in pCS2 is not the
standard T7 primer sequence, so sequencing facilities may not be
able to sequence from that end with their stock primers. I think you
need to provide the sequencing facility with a pCS2 appropriate “T7"
primer or use T3 (a little farther from the 3’ end). SP6 and T3 are
fine.

I think this plasmid might be from Eddy DeRobertis lab, so you can
also check with them about it.

Peter


On Jun 11, 2014, at 2:02 PM, Sally Moody <samoody at gwu.edu<mailto:samoody at gwu.edu>> wrote:

Hi Jill,
We don't have that sequence, but in our experience it is better to
send it out for sequencing rather than rely on somebody's map
because things are not always what they seem. Since it is in pCS,
you can use M13 primers or better yet SP6 and T7 primers for that
plasmid.
Good luck, Sally


On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston
<johnstoj at ucalgary.ca<mailto:johnstoj at ucalgary.ca>> wrote:
Hello,

We have a construct called pCS-GAP43-GFP here and have no information
about it.  Does anyone have the sequence that could be sent to us?

Thanks


Jillian Johnston
Research Associate
Dr. Sarah McFarlane lab
University of Calgary
Faculty of Medicine
Dept of Cell Biology and Anatomy




_______________________________________________
Xenopus mailing list
Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
https://lists.mbl.edu/mailman/listinfo/xenopus



--
Sally A. Moody, Ph.D.
Professor of Anatomy and Regenerative Biology
George Washington University
School of Medicine and Health Sciences
2300 I Street, NW, Washington, DC, 20037, USA
202-994-2878
Editor-in-Chief:  genesis, The Journal of Genetics and Development
Editor: Principles of Developmental Genetics, 2nd edition
_______________________________________________
Xenopus mailing list
Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
https://lists.mbl.edu/mailman/listinfo/xenopus


_______________________________________________
Xenopus mailing list
Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
https://lists.mbl.edu/mailman/listinfo/xenopus


_______________________________________________
Xenopus mailing list
Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
https://lists.mbl.edu/mailman/listinfo/xenopus
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lists.mbl.edu/pipermail/xenopus/attachments/20140611/1e13324e/attachment.html