[Xenopus] pCS-GAP43-GFP sequence

[Jill Johnston]

We use a home made T7 primer for the original CS2 vector so worries about
the result I'll get back.

Jill

> Thx!
>
> --
> Daniel C. Weinstein
> Associate Professor
> Biology Department
> Queens College, CUNY
> 65-30 Kissena Boulevard<x-apple-data-detectors://0/0>
> Queens, NY 11367-1597
> 718-997-4552<tel:718-997-4552> (office) /4258 (lab)
> Fax: 718-559-6745<tel:718-559-6745>
> daniel.weinstein at qc.cuny.edu<mailto:daniel.weinstein at qc.cuny.edu>
>
> On Jun 11, 2014, at 3:18 PM, "Takuya Nakayama"
> <tn8t at virginia.edu<mailto:tn8t at virginia.edu>> wrote:
>
> That's what I was about to point out too.
> In case you wonder, here is the "T7" primer which we've been using for
> pCS2+ sequencing: 5'-AATACGACTCACTATAG-3' (just shorter version of
> original T7).
>
> Takuya
>
>
> On Jun 11, 2014, at 2:49 PM, Peter Klein wrote:
>
> Hi Jill,
>
> I agree with Sally. But the “T7" sequence in pCS2 is not the
> standard T7 primer sequence, so sequencing facilities may not be
> able to sequence from that end with their stock primers. I think you
> need to provide the sequencing facility with a pCS2 appropriate “T7"
> primer or use T3 (a little farther from the 3’ end). SP6 and T3 are
> fine.
>
> I think this plasmid might be from Eddy DeRobertis lab, so you can
> also check with them about it.
>
> Peter
>
>
> On Jun 11, 2014, at 2:02 PM, Sally Moody
> <samoody at gwu.edu<mailto:samoody at gwu.edu>> wrote:
>
> Hi Jill,
> We don't have that sequence, but in our experience it is better to
> send it out for sequencing rather than rely on somebody's map
> because things are not always what they seem. Since it is in pCS,
> you can use M13 primers or better yet SP6 and T7 primers for that
> plasmid.
> Good luck, Sally
>
>
> On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston
> <johnstoj at ucalgary.ca<mailto:johnstoj at ucalgary.ca>> wrote:
> Hello,
>
> We have a construct called pCS-GAP43-GFP here and have no information
> about it.  Does anyone have the sequence that could be sent to us?
>
> Thanks
>
>
> Jillian Johnston
> Research Associate
> Dr. Sarah McFarlane lab
> University of Calgary
> Faculty of Medicine
> Dept of Cell Biology and Anatomy
>
>
>
>
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
> https://lists.mbl.edu/mailman/listinfo/xenopus
>
>
>
> --
> Sally A. Moody, Ph.D.
> Professor of Anatomy and Regenerative Biology
> George Washington University
> School of Medicine and Health Sciences
> 2300 I Street, NW, Washington, DC, 20037, USA
> 202-994-2878
> Editor-in-Chief:  genesis, The Journal of Genetics and Development
> Editor: Principles of Developmental Genetics, 2nd edition
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
> https://lists.mbl.edu/mailman/listinfo/xenopus
>
>
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
> https://lists.mbl.edu/mailman/listinfo/xenopus
>
>
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu<mailto:Xenopus at lists.mbl.edu>
> https://lists.mbl.edu/mailman/listinfo/xenopus
> _______________________________________________
> Xenopus mailing list
> Xenopus at lists.mbl.edu
> https://lists.mbl.edu/mailman/listinfo/xenopus
>


Jillian Johnston
Research Associate
Dr. Sarah McFarlane lab
University of Calgary
Faculty of Medicine
Dept of Cell Biology and Anatomy