[Xenopus] New transgenic lines

[National Xenopus Resource]

Dear Xenopus community 
There are several new transgenic lines available for purchase. The names of the lines and their birth dates are listed below: 

EB3-GFP (Oct. 2015) 


CMV promoter drives expression of GFP in the microtubule. 

CMV-hEB3-EGFP, consists of a cytomegalovirus (CMV) promoter, 1-281 amino acids of human end-binding protein 3 (hEB3)(Stepanova et al. 2003), and EGFP in pEGFP-N1 (Clontech) 

Takagi et al. 2013 Dev. Growth Differ 55, 422-433. 

REMI – Ueno lab 


EGFP-Golgi (Oct. 2015) 


CMV promoter drives expression of GFP in the Golgi apparatus. 

CMV-hβ4GalT-EGFP, consists of a cytomegalovirus (CMV) promoter, the N terminal 81 amino acids of human β1, 4-galactosyltransferase (β4GalT), and EGFP in pCS2p+. 

Takagi et al. 2013 Dev. Growth Differ 55, 422-433. 

REMI – Ueno lab 




Sox10-GFP (Xla.Tg(-4.7Sox10:GFP)) (Oct. 2015) 


GFP expression in the cranial neural crest cells. 

Green eyes selection marker. 
Sox10:GFP-HS4-Isce1 transgene contains 4.7Kb of upstream sequence from the transcription factor sox10 (including part of the 5’ UTR). 

This is an inert transgene label that reveals the migratory cranial neural crest cell population in early embryos through the distribution of green fluorescent protein. 

I Sce-I – Saint-Jeannet lab 




RFP-ER (Nov. 2015) 



CMV promoter drives expression of RFP in the endoplasmic reticulum. CMV-CALR-RFP-KDEL, consists of a cytomegalovirus (CMV) promoter, the calreticulin (CALR) targeting sequence, RFP, and the KDEL coding sequence in pCS2p+. 



Takagi et al. 2013 Dev. Growth Differ 55, 422-433. 



REMI – Ueno lab 




mPrx1(Prrx1):GFP (July 2015) 

GFP fluorescence in limb bud. The transgene labels mesenchymal cells in limb bud and limb blastema. CMV promoter of pCSGFP3 was removed by SalI and HindIII and a 2.4-kb genomic sequence upstream of mouse prx1 was inserted with the same restriction enzyme sites. Thus, the 2.4-kb mouse prx1 limb-specific enhancer driving GFP3 is inserted into the genome. 

Dev Biol 304 (2007) 675–686 

REMI – Tamura lab 



pbin7Lef:dGFP (Feb 2015 and Sept 2015) 

GFP expression reveals dynamic patterns of Wnt/β-catenin signalling activity. First clear signals present at neurula stage in the neural folds. The inserted tansgene construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. See Tran et al., 2010 and 2013 for a full description. 

H.T. Tran, K. Vleminckx, Methods (2013),. 

Tran, H., et. al.(2010). Acad. Sci. USA 107 (2010) 16160–16165. 

REMI – Vleminckx lab 


Other lines that will be ready to sell in a few months 



RFP-Mem (March 2016) 

CMV promoter drives expression of RFP in the plasma cell membrane. CMV-RFP-CAAX, consists of a cytomegalovirus (CMV) promoter, RFP, and the c-Ha-Ras farnesylation signal in pCS2p+. 

Takagi et al. 2013 Dev. Growth Differ 55, 422-433. 

REMI – Ueno lab 




HyPer (Xla.Tg(crag:RFP, CMV:HyPer-YFP) Amaya (March 2016) 

Ubiquitous hyper YFP expression, marker for integration selection: RFP expression in the eye. 

g-crys RFP CMV HyPerYFP. (H202 indicator) 

REMI – Amaya lab 




LMO2- Katushka (Xla.Tg(lmo2:Katushka) EXRC ) (March 2016) 

Katushka expression in the blood. 

4775bp X. tropicalis LMO2 promoter driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP. 

X. tropicalis fosmid AOPZ 60793; scaffold 82: 386828-391603. 

I Sce-I – EXRC 




Feel free to email us if you have questions 




Sincerely, 

NXR staff 


**************************************************************** 
If the NXR contributed to your work please cite us in your papers. 

Cite us in the acknowledgements: National Xenopus Resource RRID:SCR_013731 

Cite us in your references: Pearl, E. et al "Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center" Genesis 50, 155-163 


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Marko Horb, Ph.D. 
Director, National Xenopus Resource (NXR) 
Marine Biological Laboratory 
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Email: xenopus at mbl.edu 
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