[Xenopus] Use of LH as replacement for hCG
National Xenopus Resource
xenopus at mbl.edu
Thu Jun 2 15:48:23 EDT 2016
Dear Xenopus Community,
I know for many of you obtaining hCG can be problematic at times. The NXR collaborated with the National Hormone and Peptide Program ( http://www.humc.edu/hormones/ ) to examine whether there are alternatives to hCG for inducing ovulation in Xenopus. I am happy to report that we found ovine and human LH to be excellent substitutes for hCG, and that the cost was less than hCG. I know some of you have already inquired about this and several veterinarians have contacted me about this. So I would like to inform you that our paper has been accepted and is now in press. You can now cite this paper for your IACUC, and of course in your publications. Hopefully this will help many of you out. The NHPP produces highly purified hormones an I recommend them. You can find the details of dose and such in our publication:
http://www.sciencedirect.com/science/article/pii/S0012160616301543
Sincerely,
Marko Horb
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If the NXR contributed to your work please cite us in your papers.
Cite us in the acknowledgements: National Xenopus Resource RRID:SCR_013731
Cite us in your references: Pearl, E. et al "Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center" Genesis 50, 155-163
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Marko Horb, Ph.D.
Director, National Xenopus Resource (NXR)
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
Email: xenopus at mbl.edu
Office: 508-289-7627
Cell: 508-564-3764
http://www.mbl.edu/xenopus
https://twitter.com/xenopusnxr
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