[Xenopus] Xenopus Digest, Vol 113, Issue 3

[Kris Vleminckx]

Hi Richard and co,

a year and a half ago we also had fertilization and mating issues with 
our trops. These were solved when we switched from Pregnyl to Chorulon. 
We have no experience with Sigma, but yes, there seems to be 
vendor-dependent variability in HCG quality.

Good luck!

Kris

On 14/10/2022 1:05, xenopus-request at lists.mbl.edu wrote:
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>     1. Problems with HCG (Richard Harland)
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> Message: 1
> Date: Thu, 13 Oct 2022 15:49:13 -0700
> From: Richard Harland <harland at berkeley.edu>
> Subject: [Xenopus] Problems with HCG
> To: xenopus at lists.mbl.edu
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> Dear Xeno(pus)philes
> We recently switched to using Sigma HCG, and have had very unreliable and late ovulations.  Is this a general issue with Sigma HCG?  Or might this be a bad batch?
> Previously we used Chorulon, during an ordering problem, which fortunately has been overcome.
> Richard Harland
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-- 
Kris Vleminckx, PhD
Ghent University
Department of Biomedical Molecular Biology
Technologiepark 71
9052 Ghent
Belgium
Tel: +32 9 33 13 760
Email: Kris.Vleminckx at irc.ugent.be
https://www.irc.ugent.be/index.php?id=krisvleminckxhome