[Xenopus] Problems with HCG

[Caroline McKeown]

Hi Richard,
A few years ago we had trouble getting Sigma hCG so I priced out all the available options. We landed on Chorulon which costs about $4 per laevis mating pair vs Sigma which was $13 per mating pair. We also occasionally used the Harbor-UCLA NHPP (National Hormone and Protein production) which was even cheaper at $2.75 per mating pair (laevis), but they don't always have it (but they always have leutinizing hormone which is even cheaper and works too). Recently California put hCG on the DEA list so now we only buy Chorulon through our institutional vet. It works consistently well in laevis.
Hope that helps,
Caroline


Caroline R. McKeown, Ph.D.
Sr. Staff Scientist
She/Her/Hers

Cline Lab, Dept. of Neuroscience
The Scripps Research Institute
10550 N. Torrey Pines Rd., DNC-216
La Jolla, CA 92037

ph: 858-784-2219
clinelab.org

________________________________
From: xenopus-bounces at lists.mbl.edu <xenopus-bounces at lists.mbl.edu> on behalf of Darcy B. Kelley <dbk3 at columbia.edu>
Sent: Friday, October 14, 2022 7:50:32 AM
To: Richard Harland <harland at berkeley.edu>
Cc: xenopus at lists.mbl.edu <xenopus at lists.mbl.edu>
Subject: Re: [Xenopus] Problems with HCG

We are required to use Chorulon (vet approved and supplier approved; HCG is DEA regulated in NYS) and it works well so have not used Sigma recently.

Chorulon is fine.

Darcy

On Thu, Oct 13, 2022 at 7:06 PM Richard Harland <harland at berkeley.edu<mailto:harland at berkeley.edu>> wrote:
Dear Xeno(pus)philes
We recently switched to using Sigma HCG, and have had very unreliable and late ovulations.  Is this a general issue with Sigma HCG?  Or might this be a bad batch?
Previously we used Chorulon, during an ordering problem, which fortunately has been overcome.
Richard Harland

[cid:183d6f804b4aa407671]
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--
Darcy B. Kelley
Harold Weintraub Professor of Biological Sciences
Director, Doctoral Program in Neurobiology and Behavior
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