[Xenopus] Xenopus Digest, Vol 118, Issue 6

Wed Mar 15 12:26:21 EDT 2023

FYI, I recently contacted the Parker  company (ph.parker.cpm) who made the PICOSPRITZER III, a microinjection system popular with Xenopus  labs. 
Note the operative word being MADE. They discontinued this device in July 2022, so it is no longer available. 

Christina @ Xenbase

On 3/15/23, 12:00 PM, "xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu> on behalf of xenopus-request at lists.mbl.edu <mailto:xenopus-request at lists.mbl.edu>" <xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu> on behalf of xenopus-request at lists.mbl.edu <mailto:xenopus-request at lists.mbl.edu>> wrote:

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Today's Topics:

1. Re: microinjector (Henry, Jonathan Joseph)
2. Re: microinjector (Davidson, Lance)

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Message: 1
Date: Tue, 14 Mar 2023 18:30:38 +0000
From: "Henry, Jonathan Joseph" <j-henry4 at illinois.edu <mailto:j-henry4 at illinois.edu>>
Subject: Re: [Xenopus] microinjector
To: National Xenopus Resource <xenopus at mbl.edu <mailto:xenopus at mbl.edu>>,
"xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>" <xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>>
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Another possibility is the Sutter Xenoworks injector has onboard air compressor and vacuum too.

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From: xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu> <xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu>> on behalf of National Xenopus Resource <xenopus at mbl.edu <mailto:xenopus at mbl.edu>>
Sent: Tuesday, March 14, 2023 2:02:50 PM
To: xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu> <xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>>
Subject: [Xenopus] FW: microinjector

Jan tried to send this email to the list and for some reason it bounced so I am forwarding it to you all

Marko

From: Jan Christian <jan.christian at neuro.utah.edu <mailto:jan.christian at neuro.utah.edu>>
Date: Tuesday, March 14, 2023 at 1:44 PM
To: xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu> <xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu>>, National Xenopus Resource <xenopus at mbl.edu <mailto:xenopus at mbl.edu>>
Subject: microinjector

Hi,

We are needing to replace one of our Warner instruments PLI-100A microinjectors and apparently these are no longer made (replaced by the AI Roboinjector that can inject up to 4 samples per well in 386 well dishes!!!). Anyone have any suggestions for a replacement that has both fill and inject capabilities. We do a lot of blastocoele aspirations so need the fill feature.

Thanks,

Jan
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Message: 2
Date: Tue, 14 Mar 2023 20:46:42 +0000
From: "Davidson, Lance" <lad43 at pitt.edu <mailto:lad43 at pitt.edu>>
Subject: Re: [Xenopus] microinjector
To: "xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>" <xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>>
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We have had good luck with used PLI-100A from Ebay (Warner, Harvard Apparatus, Medical Systems). They remain functional and get frequent use.

Lance

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Subject: Xenopus Digest, Vol 118, Issue 5

When replying, please edit your Subject line so it is more specific than "Re: Contents of Xenopus digest..."

Today's Topics:

1. Re: microinjector (Chang, Chenbei)

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Message: 1
Date: Tue, 14 Mar 2023 18:29:51 +0000
From: "Chang, Chenbei" <cchang at uab.edu <mailto:cchang at uab.edu>>
Subject: Re: [Xenopus] microinjector
To: National Xenopus Resource <xenopus at mbl.edu <mailto:xenopus at mbl.edu>>,
"xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>" <xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>>
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We recently got an injector PM1000 from MicroData Instrument (see attached catalog). It can both fill and inject. There is only one foot pedal, so you have to toggle between fill and inject constantly. However, once you get used to it, it works quite well. It is much cheaper than Warner Instruments device. The other choice is Narishige. It is more expensive than MDI injector too.

From: xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu> <xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu>> On Behalf Of National Xenopus Resource
Sent: Tuesday, March 14, 2023 1:03 PM
To: xenopus at lists.mbl.edu <mailto:xenopus at lists.mbl.edu>
Subject: [Xenopus] FW: microinjector

Jan tried to send this email to the list and for some reason it bounced so I am forwarding it to you all

Marko

From: Jan Christian <jan.christian at neuro.utah.edu <mailto:jan.christian at neuro.utah.edu><mailto:jan.christian at neuro.utah.edu <mailto:jan.christian at neuro.utah.edu>>>
Date: Tuesday, March 14, 2023 at 1:44 PM
To: xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu><mailto:xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu>> <xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu><mailto:xenopus-bounces at lists.mbl.edu <mailto:xenopus-bounces at lists.mbl.edu>>>, National Xenopus Resource <xenopus at mbl.edu <mailto:xenopus at mbl.edu><mailto:xenopus at mbl.edu <mailto:xenopus at mbl.edu>>>
Subject: microinjector
Hi,
We are needing to replace one of our Warner instruments PLI-100A microinjectors and apparently these are no longer made (replaced by the AI Roboinjector that can inject up to 4 samples per well in 386 well dishes!!!). Anyone have any suggestions for a replacement that has both fill and inject capabilities. We do a lot of blastocoele aspirations so need the fill feature.

Thanks,
Jan

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End of Xenopus Digest, Vol 118, Issue 6
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