[Xenopus] microinjector

[Henry, Jonathan Joseph]

The only issue with used PLI 100 units is there are no spare parts.  You can only replace tubing, frequently cracked.  Harvard will not take them for service. I have a colleague who bought two cheap and assembled one working unit from those.

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From: xenopus-bounces at lists.mbl.edu <xenopus-bounces at lists.mbl.edu> on behalf of Davidson, Lance <lad43 at pitt.edu>
Sent: Tuesday, March 14, 2023 4:46:42 PM
To: xenopus at lists.mbl.edu <xenopus at lists.mbl.edu>
Subject: Re: [Xenopus] microinjector

We have had good luck with used PLI-100A from Ebay (Warner, Harvard Apparatus, Medical Systems). They remain functional and get frequent use.

Lance



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Today's Topics:

   1. Re: microinjector (Chang, Chenbei)


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Message: 1
Date: Tue, 14 Mar 2023 18:29:51 +0000
From: "Chang, Chenbei" <cchang at uab.edu>
Subject: Re: [Xenopus] microinjector
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        "xenopus at lists.mbl.edu" <xenopus at lists.mbl.edu>
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We recently got an injector PM1000 from MicroData Instrument (see attached catalog).  It can both fill and inject.  There is only one foot pedal, so you have to toggle between fill and inject constantly.  However, once you get used to it, it works quite well.  It is much cheaper than Warner Instruments device.  The other choice is Narishige.  It is more expensive than MDI injector too.

From: xenopus-bounces at lists.mbl.edu <xenopus-bounces at lists.mbl.edu> On Behalf Of National Xenopus Resource
Sent: Tuesday, March 14, 2023 1:03 PM
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Subject: [Xenopus] FW: microinjector

Jan tried to send this email to the list and for some reason it bounced so I am forwarding it to you all

Marko


From: Jan Christian <jan.christian at neuro.utah.edu<mailto:jan.christian at neuro.utah.edu>>
Date: Tuesday, March 14, 2023 at 1:44 PM
To: xenopus-bounces at lists.mbl.edu<mailto:xenopus-bounces at lists.mbl.edu> <xenopus-bounces at lists.mbl.edu<mailto:xenopus-bounces at lists.mbl.edu>>, National Xenopus Resource <xenopus at mbl.edu<mailto:xenopus at mbl.edu>>
Subject: microinjector
Hi,
We are needing to replace one of our Warner instruments  PLI-100A microinjectors and apparently these are no longer made (replaced by the AI Roboinjector that can inject up to 4 samples per well in 386 well dishes!!!).  Anyone have any suggestions for a replacement that has both fill and inject capabilities.  We do a lot of blastocoele aspirations so need the fill feature.

Thanks,
Jan


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